196 HAROLD J. COOPER 



the orange G. No special features were observed in these except 

 that they were perhaps slightly smaller than the cells staining 

 with the acid fuchsin. In these cells, as shown in figure 6, the 

 ground substance of the cytoplasm stains a clear light orange and 

 contains a great number of irregular granules which take a deep 

 orange color. 



Scaffidi, on the basis of this method of staining sections from 

 the human gland, differentiates between what he considered to 

 be two different functional types. Those taking up the orange G, 

 he states, are round, and their golden granules small, fine, and 

 glistening. They have a small oval nucleus with large chromatin 

 granules. The 'fuchsinophile' cells are described as larger and 

 more irregular and their granules irregular and coarse. The 

 nuclei are larger and generally rounded. In them occur small 

 blocks staining deeply with the fuchsin. He mentions nothing 

 about these blocks in detail except to say that they are never 

 found in the 'orange G staining cells.' These blocks I was unable 

 to find in the squirrel gland or in the human. 



In order to understand the distinction made on this basis, 

 sections of human hypophysis and adrenal, as well as the same 

 tissues from the ground-squirrel, were first stained with orange 

 G. Certain groups of cells were located with a mechanical stage, 

 studied and drawn. The preparations were then decolorized 

 and restained with acid fuchsin. In the human hypophysis, it 

 was found, practically without exception, that those cells which 

 had at first stained with the orange G, became restained with the 

 fuchsin, while those remaining colorless at the first staining, con- 

 tinued so during the second. The same was true without excep- 

 tion with the squirrel hypophysis and both adrenals. Besides 

 this set of stains, eosin and fuchsin, eosin and chromotrope 2B, 

 and fuchsin and chromotrope 2B were used with the same results. 

 These groups of stains, when used together (in a mixture) can 

 be distinguished by differences in shades, and when so used, it 

 was seen that they, too, attacked the cells somewhat differently. 

 While there is a specificity shown by different cells toward one 

 or the other of the stains in such a mixture, it does not seem to 

 offer a very sound foundation alone for a division of the cells into 

 functional individual cell types. 



