HISTOGENESIS OF BLOOD IN BUFO HALOPHILUS 



227 



The conclusions led to by the above table are as follows: 1) 

 these granules are not soluble in water after fixation, because 

 when stained for twenty-four hours they are no less distinct than 

 when they are only exposed to water for ten minutes; 2) they are 

 not azurophil, because they stain with Jenner's stain and with 

 triacid, neither of which contains any methylene azure, and 

 because they do not stain pink with azure II or Unna's poly- 

 chrome methylene blue, both of which contain methylene azure, 

 uncombined with eosin, however; 3) they are neutrophil, because 

 they do not stain with eosin, or with basic analines alone, but 

 do stain with neutral stains; 4) they have, however, a special 

 affinity for acid fuschin, for they stain red with triacid rather 

 than purplish pink. 



My special cells are without doubt identical with Mietens' 

 ('10) oxyphil wander-cells. Mietens' failure to observe granules 

 in these cells can be attributed to inappropriate fixation. Max- 

 imow ('10) speaks of special granulocytes and of special leucocytes 



