354 S. SAGUCHI 



respects, differences are observed between the two. In the prep- 

 arations fixed in formalin, sublimate-formalin, alcohol, bichro- 

 mate-formalin, Zenker's, osmium-sublimate, etc., and stained 

 after Altmann, the side-nucleolus can be more easily decolorized 

 then the main-nucleolus. In the preparations fixed in sublimate 

 and stained with iron-haematoxylin, on the contrary, the former 

 stains more deeply than the latter and exhibits also a more 

 heavily stained cortical layer (fig. 2). The side-nucleoli have no 

 structures in their interior. They are, in most cases, situated in 

 the region intermediate between the main-nucleolus and the 

 nuclear membrane, but it is not uncommon to see side-nucleoli 

 closely apposed to the main-nucleolus (fig. 8), or appearing to 

 be constricting off of the main-nucleolus (figs. 5, 6). The latter 

 fact strongly suggests the derivation of the side-nucleoli from the 

 main-nucleolus. 



c. Nucleolar corpuscles. Under this appellation I include cer- 

 tain small corpuscles in the nucleolus, which are stained black, 

 as seen in the preparations fixed in sublimate, alcohol, or subli- 

 mate-formalin, and stained with iron-haematoxylin (figs. 1 to 4, 

 7, 15, 16); in those fixed in sublimate-acetic acid, Champy's, or 

 Meves' fluid, they stain dark blue with crystal violet, and red 

 with acid fuchsin or safranin (fig. 20). They are either small 

 granules or rods of all shapes and sizes, which seem to vary with 

 the individual nuclei and the properties of the fixatives employed. 

 They are not only scattered through the nucleus, but also are in 

 close apposition to the nucleolus or to the inner surface of the 

 nuclear membrane. 



It is a difficult matter definitely to determine the relation which 

 might exist between the position of the chromatin granules or 

 cords and the nuclear corpuscles; for they cannot be brought into 

 view by staining in one and the same preparation. They, in 

 fact, differ from each other in staining reactions. In the prepa- 

 rations preserved in Champy's or sublimate-acetic mixture, and 

 stained with Benda's crystal violet, for example, the nucleolar 

 corpuscles take on a dark violet color, while the chromatin cor- 

 puscles are left unstained (figs. 10, 11, 17, 18). That the nucle- 

 olar corpuscles are, however, attached to the chromatin network, 



