386 S. SAGUCHI 



ference between the observations of some investigators (Regaud 

 and Mawas, Hoven, 0. Schultze) , who believe in the derivation 

 of zymogen granules from the mitochondria, and my own find- 

 ings. They believe to have found the disintegration of the chon- 

 dricontes and the increase in volume of the granules thus formed 

 up to the zymogen granules, in those preparations which are 

 usually employed to exhibit the mitochondria, such as Benda's, 

 Meves', etc. I have, however, never been able to follow such a 

 direct transition between the two in the above preparations ; but 

 there are granules intermediate between the mitochondria and 

 the definitive zymogen granules. These granules, prozymogen 

 granules are, therefore, different in behavior to fixing and 

 staining reagents from the two other constituents of the cell. 

 Secondly, it seems from the descriptions and drawings of the 

 above investigators that there is no locality of predilection for 

 the disintegration of the chondriocontes ; whereas, in my cases, 

 the process takes place in the secretogenous area only. 



So far as I have been able to ascertain, there was no note- 

 worthy relation between the shape, structure, and chromaticity 

 of the nucleus and the elaboration of secretion granules ; in 

 spite of the great accumulation of the latter, there could not 

 be seen any considerable flattening of the nucleus, although 

 it is often pressed down toward the basis of the cell. The 

 mitochondrial filaments are usually more numerous in empty 

 cells (fig. 55) ; this would seem to indicate that their passing 

 out of the nucleus mainly takes place in such cells. In fact, 

 the connection between the chondriocontes and the nuclear 

 network can easily be seen in such empty cells; but it is not 

 rare, that it can be found in cells heavily laden with secreting 

 granules. These facts are in essential harmony with the find- 

 ings above mentioned that the nucleus exhibits no visible changes 

 in the structure and staining reactions according to the stages 

 of secretion; in other words, the mitochondria are continually 

 supplied from the nucleus as they are used up in the formation 

 of zymogen granules. 



4. Extrusion. Concerning the question as to whether zymogen 

 granules are eliminated as such or after undergoing certain 



