388 S. SAGUCHI 



E. Intracellular net and canalicular apparatus 



Historical. The existence of canaliculi or vacuoles in the 

 glandular cell has often been noticed by various investigators. 

 Laserstein ('94) describes clear paths in the granular zone 

 of the pancreatic cell as canaliculi running through the cyto- 

 plasm. E. Muller's investigation ('98) of the gastric fundus 

 gland has drawn attention to these structures. These cells con- 

 tain, according to this author, a network of clear distinct bands 

 which are connected with the intercellular secretion capillary, 

 and which are nothing other than the secreting matter formed 

 within the cell; he identified the network with a structure pre- 

 viously found by him by means of Golgi's method and named 

 'Korbkapillaren.' These observations have been confirmed by 

 Zimmermann ('98). The latter describes the intracellular secre- 

 tion capillaries as limited to the gastric fundus gland, the sweat 

 gland, and the liver, in contradistinction to the view of Krause 

 ('95), who had found canaliculi both in serous and mucous 

 cells of the salivary gland. 



The existence of a typical network in glandular and epithe- 

 lial cells, on the other hand, has been recorded at some length 

 by Negri ('00). He has demonstrated the network of the 

 pancreas cell by means of Velatti's mixture and found it inde- 

 pendent of the intercellular secretion capillaries impregnated 

 at the same time. Similar networks or canaliculi have since been 

 described by Holmgren ('02), Bergen ('04), Babkin, Rubasch- 

 kin and Ssawitsch ('09), Kolster ('13), Bensley ('11), and 

 others in the pancreatic cells of various species. 



Observations. So far as I have been able to determine, there 

 can be made manifest, in the pancreatic cells of Rana temporaria, 

 either networks consisting of solid cords or canaliculi, according 

 to the technique employed. Although both pictures seem to 

 belong to one and the same structure, as will be afterward 

 explained, they are conveniently treated here separately. 



1. Intracellular network, a. Technique. By 'intracellular 

 network' we mean the apparatus which can be made manifest by 

 various methods, such as Kopsch's, and Weigl's osmium method, 



