404 S. SAGUCHI 



As already mentioned, these fibrillar structures when pre- 

 served in alcohol, sublimate mixtures, etc., stain with alum-hae- 

 matoxylin a blue color, as is the case with the chromatin. From 

 this staining reaction alone, however, it cannot be inferred that 

 they are derived from the nucleus, as assumed by some; for, if 

 the stains, such as safranin, iron-haematoxylin, etc., are em- 

 ployed, they are easily decolorized, whereas the chromatin i& 

 heavily stained. 



b. I have before mentioned that the physiological degenera- 

 tion of the cell is characterized by chromatic separation followed 

 by constriction of the nucleus and the cell-body, and that the 

 spherical fragments produced by the latter process and taken up 

 by the neighboring glandular cells exhibit every variety of struc- 

 ture. The nuclear portion in the fragment, when it exists, is 

 variable, and is either spherical or crescentic, or ring-formed 

 according to its size and the phase of the nuclear change; while 

 the cytoplasmic portion, small or large in amount, either contains 

 unaltered zymogen granules or may be entirely devoid of them. 

 There are also many corpuscles which contain no nuclear 

 fragments. 



These corpuscles are to be identified with those mebenkerne r 

 which I have before summarized under the second type. The 

 'nebenkerne' which belong to the second type are spherical in 

 shape and have an even contour ; they are surrounded with a 

 clear halo ; in other words, they lie in vacuoles of the cytoplasm. 

 This fact shows that these corpuscles are not closely related to 

 the cytoplasm containing them ; it is therefore not without reason 

 that they have been supposed by some to be parasites passed 

 into the cell. That these 'nebenkerne' cannot be interpreted as 

 anything else than corpuscles produced by the fragmentation of 

 the degenerated cell will be evidenced by considering their struc- 

 ture. In the following I will try to criticise the observations of 

 various investigators, comparing the various types of 'nebenkerne' 

 with our fragments. 



First, in figures 4, a, d, e, and 5, a, accompanying Ogata's 

 paper, it can clearly be seen that these corpuscles are nothing 

 other than nuclei, which have already suffered chromatic 



