MITOCHONDRIA (AND OTHER CYTOPLASMIC 

 STRUCTURES) IN TISSUE CULTURES 



MARGARET REED LEWIS AND. WARREN HARMON LEWIS' 



From the Anatomical Laboratory, Johns Hopkins Medical School, and the Marine 

 Biological Laboratory, Woods Hole, Mass. 



TWENTY-SIX FIGURES 



CONTENTS 



Introduction 34O 



Technic 34I 



Fixation 344 



Mitochondria 347 



Position of mitochondria 348 



Shape of mitochondria 352 



Size of mitochondria 359 



Number of mitochondria 360 



Quantity of mitochondria 360 



Relation between position, size, number and quantity of mitochondria. . . 362 



Degenerate mitochondria 363 



Mitochondria in mitosis 365 



Mitochondria in different kinds of cells 372 



Experimental work 373 



Reaction to acids 373 



Reaction to alkalies 374 



Reaction to xylol, chloroform, ether 374 



Reaction to hyper and hypotonic solutions 374 



Reaction to heat 374 



Vital dyes 376 



Janus green 376 



Nile blue B extra and brilliant cresyl blue 2b 377 



Iodine 379 



Certain other cell structures and their relation to the mitochondria 380 



Granules 380 



Vacuoles 381 



Fat globules 386 



Canalicular system 390 



Amitosis and giant cells 391 



Discussion 392 



Conclusion 394 



Bibliography 397 



1 We are indebted to the Marine Biological Laboratory for the use of a room 

 during the summer of 1914. 



339 



