MITOCHONDRIA IN TISSUE CULTURES 



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about the nucleus and central body; the dividing cell has 118 mitochondria which 

 are scattered more evenly through the cytoplasm than in the other cells; X 790 

 diam.; osmic acid vapor and iron hematoxylin; /, cell from a 2-day culture of 

 heart from a 6-day chick cell with 152 mitochondria, mostly rod- and thread- 

 shaped, arranged about nucleus and central body; X 790 diam. g, cell from a 

 2-day culture of heart from a 4-day chick. Only two cells in entire culture show 

 these ring-formed mitochondria and in these two the cytoplasm was abnormal, 

 perhaps dead. Bensley stain; X 790 diam. 



Fig. 5 A, b, c, d, four adjoining cells from a 2-day culture of heart from a 

 5-day chick; X 790 diam. The resting cells, a, b and c, have 47, 51 and 48 mito- 

 chondria respectively, while cell d in early prophase has 89. These cells exhibit 

 great variety in the shapes of the mitochondria; small and large granules, spin- 

 dles, short rods, long rods and threads are present; the mitochondria are some- 

 what more scattered than the cytoplasm in cell d. e, cell with 38 mitochondria 

 which vary greatly in size and shape; from the same specimen; X 540; osmic 

 acid vapor and iron hematoxylin. /, g, mesenchyme cells from 2-day cultures 

 of intestine from a 7-day chick embryo ; /, many of the mitochondria are united 

 in networks; X 540 diam.; g, some ot the mitochondria branch and anastomose 

 to form a complicated network which appears to extend from one nuclear area 

 to another, where the cells form a syncytium; X 790 diam. ; only a portion of the 

 cells and network is shown; osmic acid vapor and iron hematoxylin. 



