380 MARGARET R. LEWIS AND WARREN H. LEWIS 



mitochondriiun nor within the loop or ring shaped mitochondria. 

 A few glycogen granules were occasionally present, however, 

 as could be distinguished by the color reaction. The fat glob- 

 ules stain first a pale port wine color which later becomes black- 

 ened. If Guilliermond's ('13) conclusion that the loop-shaped 

 mitochondria give off glycogen granules is correct, one would 

 certainly expect to find that iodine used in connection with un- 

 fixed material would show this at least during the final stage 

 in the formation of the glycogen when the granule lies free but 

 still in the neighborhood of the mitochondrium from which it 

 came. 



CERTAIN OTHER CELL STRUCTURES AND THEIR RELATION TO THE 



MITOCHONDRIA 



Granules 



Certain other granules were present in most of the cells of 

 these growths, but so far, these granules have not been carefully 

 studied. They can be differentiated from mitochondria of simi- 

 lar shape by the greater rapidity with which the granules move 

 through the cytoplasm. Certain of the vital dyes which color 

 these granules leave the mitochondria unstained. Neutral red 

 usually stains one or several granules near the central body. 

 Nile blue B extra and brilliant cresyl blue 2 b also stain certain 

 granules near the central body. In cells which contain the body 

 we have termed vacuole (see below) one or more of these granules 

 are present within the vacuoles and are stained blue within a 

 pink vacuole (nile blue B extra) or purple within a pink vacuole 

 (brilliant cresyl blue 2 b) . These granules are few in number in 

 the normal cell but plentiful in cells which contain many vacuoles. 

 Other vital dyes stain certain granules within the cell, but so 

 far as our observations go they are the same as the neutral red 

 granules or else as the nile blue, and brilliant cresyl blue granules. 



At times the granules within the vacuole take the Janus green 

 color as a very pale green, but no other relation between these 

 granules and the mitochondria has been found. 



