382 



MARGARET R. LEWIS AND WARREN H. LEWIS 



the side. These granules usually stain a pale green with Janus 

 green stain. 



Nile blue B extra and brilliant cresyl blue 2 b each act as a 

 differential stain for these bodies. The vacuole stains pink and 

 the granules blue (nile blue B extra) or purple (brilliant cresyl 

 blue 2 b, fig. 23). 



vac. a. i*) W ■ GD 



10.46 A. M 



10.48 A.M. 



^>>. 





Jr 



'r;^ 



©0© & ^ <s^<^® ® 



IIA.M. 11.5A.M. 11.12A.M. 



\- - - granule 

 X 



11.30 A.M. 



b 



1.5 P.M. 



XII A.M. 



C \ 



X 11.5 A.M. 



d 



X 12 Noon 

 O vac. b. 11.20 A.M. 



V 



12.2 P.M. 



V \Nxii.8am. 



^ X 12.30 P.M. 



O 



Op. 



o, 



=e) 



=.b.O o © o • / t 



11.20 A.M. 12 Noon * 12.; 



20 P.M. 



12.45 P.M. 



r t 



h 



y / . . , 



I 



o o © o (9 3 y 



1.45 P.M 



1 P.M. 



Fig. 23 Observations on the behavior of vacuoles in the living cell with 

 brilliant cresyl blue 2 B (see text). 



