384 MAEGARET R. LEWIS AND WARREN H. LEWIS 



12.30 A.M. The granule shows no sign of pink vacuo^.e or streamer 

 and remains quiet near the nucleus. 



11.20 A.M. A clear unstained vacuole (vac.b.) appeared in the cyto- 

 plasm between two of the purple granules (fig. 23 g) and behaved as 

 follows, (fig. 23 h). 



11.30 A.M. It became a pale violet vacuole with a few dancing un- 

 stained granules. 



11.45 A.M. It was a violet vacuole with purple granules. 



11.50 A.M. Violet vacuole became pink with purple granules. 



12.00 NOON. The vacuole condensed into a small bright pink vacuole 

 with only one purple granule. 



12.13 P.M. The vacuole entirely disappeared and only the purple 

 granule remained. 



12.20 P.M. Purple granule sent out a pink streamer. 



12.45 P.M. The pink streamer osculates and is rapidly sent out and 

 drawn in again. 



1.45 P.M. The granule became quiet and the streamer disappeared. 

 The purple granules later moved as a rod from the periphery of the 

 cell in towards the nucleus and back S3veral times. It passed over 

 and under the mitochondria without hindrance. Other purple gran- 

 ules in the cell moved rapidly without streamers, some as double gran- 

 ules, others as rods or as single round granules. 



12.10 P.M. A pale space appeared in an adjoining cell (fig. 23 i). 



12.15 P.M. This space became pale violet. 



12.20 P.M. Violet color changed to pink. 



12.25 P.M. Granules appeared in the vacuole. 



12.27 P.M. Granules became deep purple granules. 



The mitochondria and the fat globules remain unstained in all the 

 cells of the growth. In some other cells of the growth many pink vacu- 

 oles are present and also many purple granules. In such cells the 

 mitochondria are mostly small granules and only a few rod- or thread- 

 like ones remain. In the cell under observation the mitochondria did 

 not change type although they were continually changing shape. 



There was no direct connection between the mitochondria 

 and the formation of the vacuoles in the above observation, and 

 yet in many cells there is often a coincident change in the shape 

 of the mitochondria until in cells which contain many vacuoles 

 within the cytoplasm the mitochondria are no longer in the 

 shape of rods and threads but then appear as small granules. 



As stated above, the fixed and stained preparations (f g. 24 a) 

 do seem to show all stages in the formation of the vacuoles 

 from the mitochondria just as Dubreuil and Guilliermond have 

 shown the formation of bodies from the mitochondria. How- 

 ever, the fact that the vacuoles have been observed to arise 



