MITOCHONDRIA IN TI8SUE CULTURES 



385 



independently of the mitochondria, although there is a coincident 

 change in the shape of the mitochondria makes one exceedingly 

 wary of accepting any evidence from the fixed and stained prep- 

 arations in this regard without cori-oboration from observations 

 upon the living cell. 



e 



// 



Fig. 24 One-day culture of heart from a o-day chick embryo; a, cell with 

 various shaped mitochondria similar to those figured by Dubreuil and Ciuillier- 

 mond, which led them to conclude that mitochondria formed fat and other 

 bodies. Observations on this cell while living gave no evidence for the forma- 

 tion of such bodies from the mitochondria; h and c show successive forms of 

 two mitochondria from the above cell and d also shows changes exhibited by a 

 single mitochondrium that Guilliermond might have interpreted as showing the 

 formation of a droplet; e, the rod-shaped mitochondrium which is applied closely 

 to the vacuole was observed, while the cell was living, to migrate from some little 

 distance to the vacuole; it had no connection with the formation of the vacuole. 

 If the specimen had been fixed to show the condition, as in e, one might have 

 concluded that the mitochondrium had something to do with the formation of 

 the vacuole or droplet. 



Certainly the mitochondria are intimately connected with any 

 change in the cytoplasm, often as in the case of heat without 

 manifestation of change by other bodies in the cj^toplasm, and 

 it is probable that any change which takes place in the cyto- 

 plasm such as would cause the formation of vacuoles or other 

 bodies would also have an influence upon the mitochondria of 

 that cell. 



