MITOCHONDRIA IN TISSUE CULTURES 



389 



size or to change their shape. During mitosis they remain 

 stationary and all may pass over to one daughter cell or part 

 to one and part to the other daughter cell. No relation between 

 these fat globules and the mitochondria was observed. Cells 

 which contain one or no fat globules often contain loop or ring 

 shaped mitochondria, but prolonged observation of these has 

 not shown any increase in the amount of fat. The third type 

 of fat is that of an accumulation of fat droplets in many of the 



N. 



Fig. 26 -1, h, cells from a 2-day culture of heart from a 10-clay chick in which 

 fat is accumulating; a was studied while living, after staining with nile blue 

 B extra, after osmic acid vapor, after nile blue B extra again, Sudan III and 

 Bensley's aniline fuchsin methylene green. No relation could be found between 

 mitochondria and the formation of fat; h, a typical wandering fat cell with many 

 small granular mitochondria about the fat droplets; c, cell from a 2-day culture 

 of heart from a 11-day chick, accumulating fat; prolonged fixation with osmic 

 acid followed by iron hematoxylin; the fat appears as dark granules. 



cells of a preparation due to some unknown cause (fig. 26 a, c). 

 These cells accumulate fat droplets from day to day, and some 

 cells may become crowded full of fat droplets within forty-eight 

 hours. Such cells should show the relation between the mito- 

 chondria and the fat globules were such a relation present, but 

 so far as our observations go none such could be established. 



These three types of droplets are undoubtedly fat. It is 

 possible to treat the same cell with various fat stains in succes- 



