1382 . DELLA DRIPS 
seem to have no definite arrangement. Among them may be seen 
numerous fibroblasts and endothelial cells. These are most numerous 
about the periphery of the central mass of blood. Some are already 
making their way intoit. The cells all seem to have their axes directed 
radially as if they were approaching the central mass of blood from the 
periphery of the luteal body. The luteal cells are of various sizes and 
shapes. Some are spindle-form and some polygonal, but the majority 
are spherical or oval. There is one specific characteristic of all young 
luteal cells and that is the existence of spherical granules in their pro- —- 
toplasm. In the sections fixed in Bensley’s acetic osmic bichromate 
and stained with acid fuchsin and methyl green, these granules are 
strikingly brought out (fig. 26). They are colored a brilliant red. 
Their sizes vary somewhat, but they are all spherical. The protoplasm 
of some cells is so full of these granules that it resembles a homogeneous 
red secretion, but on examination with very high power, the separate 
granules may be seen. In many cells where the granules do not fill 
the protoplasm, they are grouped about the nucleus, leaving a narrow 
clear zone about the periphery of the cell. The nuclei take the green 
stain and are strikingly brought out against the red granules. Each 
nucleus contains one or two good-sized bright, red staining nucleoli. 
The chromatin threads stain green and do not show very well with 
this stain. For the nuclear characteristics, another ovary stained 
differently will be described. 
The two abnormal luteal structures of this ovary are very interesting. 
One of them is a luteal cyst (fig. 23). There is only a single layer of 
luteal cells next to the thin capsule. No fibroblasts or endothelial 
cells are present. The whole body is filled with a transparent greenish- 
colored fluid which has every appearance of being of the same compo- 
sition as the liquor folliculi. 
The other structure (fig. 21) is much more normal. The center con- 
tains what resembles the above green-tinged fluid containing numer- 
ous red blood corpuscles. Masses of normal luteal cells are found most 
of the way around the body. But on one side is a mass of follicle or 
granulosa cells persisting untransformed. In several places, as in 
figure 22, some granulosa cells are found among the luteal cells. This 
peculiar luteal structure appears to give striking evidence to the theory 
that luteal cells are simply transformed granulosa cells. The differ- 
ences between them are well brought out in the picture. | 
It may be well to state here that other fixatives and stains bring 
out these same specific luteal-cell characteristics, particularly the 
granules in the protoplasm. With a formalin zenker fixative and the 
acid fuchsin and methyl green stain, the granules appear the same in 
every way except in color. They are pink instead of red. With this 
same fixative and a copper-chrome hematoxylin stain, the granules 
appear brownish-black. With an acetic osmic bichromate fixative and 
the copper-chrome hematoxylin stain, the granules appear bluish-black. 
The nuclear characteristics of the early luteal cells are best brought 
out with a formalin zenker fixative and a hematoxylin and eosin stain. 
