140 DELLA DRIPS 
protoplasm is absolutely full of a mass of lipoid droplets. This is seen 
to advantage in the unstained sections. In the stained sections the 
cells appear more or less honeycombed, according to the amount of 
lipoid which has been dissolved out. These lipoid droplets are very 
uniform in size and are dark brown, quite a different color from the 
black fat droplets of fatty degeneration. The red granules are gone. 
Where there is any protoplasm visible, it appears granular and pinkish- 
gray in color (fig. 28). The nuclei of the cells appear slightly smaller 
than formerly, but this apparent decrease in size is evidently due to 
the increase in the size of the cells, for the nuclei are no smaller by 
measurement. The location of the nucleus in the cell is the same as 
described under spermophile 371, either in the center or to one side of 
the center. The nucleolus stands out large and bright and the chro- 
matin strands appear as they did. There are no apparent degenerative 
changes. Beside the three corpora lutea in this ovary, there are six 
or seven medium-sized normal growing and three atretic follicles. 
There are no large follicles or even any of good size. A few primordial 
ova are present, fewer than in any of the ovaries described thus far, 
and no interstitial cells can be distinguished as such (fig. 15). 
Experiment 439-16 (spermophile 436). Captured June 23, 1916. 
Sacrificed July 15, 1916. Weight, 194.2 grams. 
Gross observations. The uterus still shows several tiny white spots 
marking the placental sites. It is otherwise normal. The ovaries 
appear to contain corpora lutea, but these latter are certainly much 
reduced in size over those of spermophile 416. They appear congested 
or of a reddish-yellow color. 
Microscopie observations of the left ovary. Fixative, acetic osmic 
bichromate. Stain, acid fuchsin and methyl green. The corpora 
lutea are much smaller (fig. 20). They measure 0.7 mm. x 0.8 mm. 
and 0.9 mm. x 0.9mm. The cells are smaller. The protoplasm of 
the cells contains no red granules and very few lipoid droplets or any 
honeycombing suggestive of these. It has a grayish, granular appear- 
ance. Something is present, however, which has not been seen before, 
and that is fat. Scattered here and there thoughout the luteal struc- 
ture, fat globules, characteristic of fatty degeneration, are present in 
the protoplasm of the cells. They are of various sizes and take on a 
characteristic black color with the osmic acid in the acetic osmic bichro- 
mate fixative. The nuclei of the cells show degenerating changes. 
The nucleolus has disappeared in some cells and in others appears 
pale and fringed. The chromatin strands are fewer and appear clumped 
in some cells. One striking new feature in the luteal structure is the 
great increase in the size of the blood-vessels and capillaries. The con- 
gestion of blood is not common to the whole ovary, but is only in the 
corpus luteum. The vascular change is apparently one factor in the 
disappearance of the luteal body. 
Experiment 515-16 (spermophile 458). Captured August 18,1916. 
Sacrificed August 21, 1916. Weight, 190 grams. 
Gross observations. There are no evidences of placental sites in the 
uterus. There are no signs of corpora lutea in the ovaries. 
