THE OVARY OF THE SPERMOPHILE 145 
Experiment 294-16 (spermophile 329). Captured May 4, 1916. 
Weight, 117 grams. Operated on May 6, 1916. The uterus was 
removed to the cervix. 
Gross observations. The animal appears to be in early pregnancy. 
Sacrificed May 20, 1916. Weight, 130 grams. 
Gross observations. There are no adhesions about the ovaries; 
they appear to contain several white, transparent corpora lutea. 
Microscopic observations of the right ovary. Fixative, formalin 
zenker. Stains, hematoxylin and eosin, and acid fuchsin and methyl 
green. There is no apparent pathologic condition of this ovary. Even 
the corpora lutea appear normal. They have proceeded in their 
development without the uterus and now have the same appearance 
as others of the same age. The only detectable difference might be a 
smaller number of red granules in the luteal cells. 
Experiment 251-16 (spermophile 348). Captured May 6, 1916. 
Weight, 108 grams. Operated on May 9, 1916. The uterus was 
removed to the cervix. 
Gross observations. The animal is pregnant. There are five 
placental swellings in each horn, measuring 6.5 mm. in length. 
Sacrificed May 19, 1916. Weight, 102 grams. 
Gross observations. Everything in the abdominal cavity is in 
excellent condition. 
Microscopic observations of the ovaries. Right ovary: fixative 
acetic osmic bichromate; stain, acid fuchsin and methyl green. Left 
ovary: fixative, formalin zenker; stain, Weigert’s copper-chrome 
hematoxylin. The ovaries are apparently normal. The corpora lutea 
appear like those of the controls except that the luteal cells contain a 
larger number of lipoid droplets and fewer red granules. There are no 
signs of degeneration in the nuclei of the cells. 
Experiment 314-16 (spermophile 349). Captured May 6, 1916. 
Weight, 122 grams. Operated on May 9, 1916. The uterus was 
removed to within 3 mm. of the cervix. 
Gross observations. The animal is pregnant. There are four 
placental swellings in the right horn and five in the left about 4 mm. 
in length. 
Sacrificed June 5, 1916. Weight, 135 grams. 
Gross observations. Everything in the abdominal cavity is in 
excellent condition. Both ovaries were readily found and appear to 
contain large corpora lutea. 
Microscopic observations of the ovaries. Right ovary: Fixative, 
formalin zenker; stain, Weigert’s copper-chrome hematoxylin. Left 
ovary: fixative, acetic osmic bichromate; stain, acid fuchsin and 
methyl green and Weigert’s copper-chrome hematoxylin. There is no 
apparent abnormality in the ovaries. The corpora lutea have devel- 
oped normally. They are still in the red-granule stage. The cells 
contain some lipoid droplets, but are full of red granules. The lipoid 
droplets in these ovaries are not as numerous as would be expected, 
but normal ovaries vary somewhat as to the time when the lipoid 
droplets begin to appear in the cells. 
