158 DELLA DRIPS 
Microscopic observations of the ovaries.. Left ovary: fixative, acetic 
osmic bichromate; stain, acid fuchsin and methyl green. The right 
ovary was lost. The left ovary contains five corpora lutea, measuring 
0.7 mm. x 0.8 mm., and showing many degeneration changes. These 
are most apparent in the corpora lutea and the clumps of interstitial 
cells of the stroma. The latter cells appear swollen and their nuclei 
are undergoing chromatolysis. The cells of the corpora lutea are most 
degenerated in the center of the structure. Some cells seem to have 
disappeared entirely here, leaving holes. Many are undergoing fatty 
changes. Toward the outside of the structure there are cells which are 
still in fairly good condition, still containing red granules, though their 
nuclei have not taken the stains well, have indented margins and 
clumped chromatin strands. 
Experiment 308-16 (spermophile 343). Captured May 6, 1916. 
Weight, 110 grams. Operated on May 8, 1916. The ovary and the 
uterus were removed. 
Gross observations: The animal is pregnant. There are eight pla- 
cental swellings in the left horn and one in the right, all 6.5 mm. in 
length. 
Sacrificed May 31, 1916. Weight, 153 grams. 
Microscopic observations of the ovaries. Right ovary: fixative, 
formalin zenker; stains, hematoxylin and eosin, Weigert’s copper- 
chrome-hematoxylin, Mallory’s connective-tissue stain. Left ovary: 
fixative, formalin zenker; stains, hematoxylin and eosin, Weigert’s 
copper-chrome-hematoxylin. The right ovary contains one corpus 
luteum, measuring 0.8 mm. x 0.8 mm., and is of the red-granule stage. 
The left ovary shows no apparent pathology. The corpora lutea meas- 
ure 1 cm. x 0.9 mm. and are of the early lipoid stage. They have 
developed normally. 
Discussion and summary of results. In four of these animals 
the remaining ovary with its corpora lutea was not at all affected 
by the operation. In two of the spermophiles operated on, the 
second ovary showed changes. In spermophile 330 the whole 
ovary was affected, and in spermophile 308, only the corpora 
lutea. There was evidently some injury to the blood supply. 
Series 8. Studies on the producion of the-normal bursting of follicles 
and the formation of corpora lutea 
Experiment 311-16 (spermophile 346). Captured in the spring of 
1916. Weight 88 grams. This animal appeared to be in rut on May 
7, 1916. She was placed with a male at 10:10 a.m. on this date and 
removed at 3 p.m. on May 8, 1916. 
Sacrificed May 8, 1916. 
