SUPRARENAL GLAND—EFFECTS OF INANITION 247 
droplets are as small as some of the liposomes. With Herx- 
heimer’s scarlet red, the liposomes stain a deep scarlet-reddish 
color, while the extracapsular ordinary fat droplets assume a 
characteristic lighter reddish color. With osmic acid, the or- 
dinary extracapsular fat stains jet-black, the suprarenal liposomes 
stain in varying shades of brown (mostly light brown). 
In distribution the liposomes, as noted in the fresh sections, 
are nearly uniformly present throughout the cortex, although 
relatively few occur in the narrow clear band between the outer 
and middle zones. In size they vary from extremely fine to 
coarse. The largest, however, are much smaller than later, 
now rarely reaching half the nuclear diameter. They form in 
each cell a circumnuclear zone which appears very distinct in 
the scarlet red or osmic-stained sections. The liposomes in 
the cortical cell strands through the medulla of the newborn 
rat usually appear similar in size and number to those in the 
cortical cells elsewhere. No liposomes are present in the cells 
of the medulla proper, which appear as clear and unstained 
masses, intermingled with the cortical strands. 
In a rat of the second day (F. 10.2), the cortical liposomes 
in frozen sections stained with scarlet red appear in general 
as in the new-born, possibly slightly more abundant in the middle 
zone. The cortical cell strands in the medulla, however, are 
apparently undergoing absorption, and their liposomes appear 
fewer and finer than elsewhere in the cortical cells. At three 
days (fig. 2), the cortical cell strands throughout the medulla 
are very inconspicuous, though still distinguishable under high 
power in many places by their content of very fine (rarely coarser) 
liposomes. In some places the corticomedullary border is now 
clearly defined, though in other places still irregular. The 
exact date at which the cortical cell strands in the medulla are 
absorbed, leaving a clean-cut corticomedullary boundary, is 
subject to individual variation. 
Second week. In rats one week of age, in sections of the su- 
prarenal stained with hematoxylin-eosin, under low magnifica- 
tion, the cortical zones appear as in the new-born, but the me- 
dulla is much lighter in appearance and now appears separated 
from the cortex by a sharply defined border. 
