SUPRARENAL GLAND—EFFECTS OF INANITION 257 
that of sebaceous gland cells. In cells with fewer lipoidal vac- 
uoles, the eosinophile granules are more conspicuous. Occa- 
sionally basophilic granules also occur. These are especially 
evident in preparations stained with iron-hematoxylin, and prob- 
ably in part correspond to the ‘corps sidérophiles’ or the mito- 
chondria described by various French investigators. The nuclei 
are usually central in position, spherical, and typical in structure. 
A few degenerative cells occur as heretofore. 
The inner zone (fig. 8, J) appears in general similar to that at 
eight weeks, the outer cells of the zone being transitional to the 
middle zone and the inner cells (next to the medulla) being typi- 
cally more or less atrophic in structure. Atrophic or degenerative 
changes have likewise been described in the inner cortical zone 
of various animals (including the rat) by Gottschau (’83), Pfaund- 
ler (92) Soulié (’03) and others. According to Kolmer (12 a, 
12 b) these degenerative changes are increased by pregnancy 
(guinea-pig). 
Pigment cells in the inner cortical zone of the rat at ten w pole 
occur more frequently than at eight weeks, but in variable de- 
gree. They are somewhat variable in number, often numerous, 
especially in the older rats, and may even extend somewhat into 
the middle zone. The nuclei of the pigment cells may be central 
in position, surrounded by the light yellowish-brown or greenish 
yellow granular masses. In this case the nucleus is irregular in 
form, but it is often pushed aside and flattened. The pigment 
is still visible, though inconspicuous, in stained paraffin sections. 
In thin sections the pigmented substance frequently appears 
vacuolated (fig. 8, P). In fresh frozen sections the pigment cells 
are clearly visible, and they are deeply stained by osmic acid or 
scarlet red. This would indicate that the pigment is a lipochrome, 
probably derived by a transformation of lipoids or other fatty 
substances. The vacuoles probably represent untransformed 
lipoids, observed by Ewald (’02) and Da Costa (’13). 
The cortical fats (lipoids) in general are best studied in frozen 
sections of suprarenals, either fresh or hardened a few hours in 
formalin. In the unstained sections the distribution of the lipo- 
somes is clearly evident on account of the varying degrees of 
