260 Cc. M. JACKSON 
The stroma (also that in the inner cortical zone) stains indistinctly 
bluish with Mallory’s anilin-blue connective-tissue stain. The 
parenchyma cells (fig. 8, M) are large. With Zenker’s fixation 
and hematoxylin-eosin stain, the cytoplasm contains the usual 
faintly basophilic (chromaffin?) granules and occasionally a few 
spherical eosinophile bodies of variable size. The cell periphery 
frequently contains irregular vacuoles, non-lipoidal and of un- 
known significance. They are variable in size and are somewhat 
more prominent than in earlier stages. According to Ciaccio 
(05), they are acidophile in reaction, like true nucleoli, and 
are comparable to ‘plasmosomes.’ The structures described by 
Ciaccio probably correspond to the eosinophile bodies referred 
to above, and not to the characteristic vacuoles. The nuclei, as 
heretofore, are typically vesicular, though a few of them are 
small and hyperchromatic (sometimes pycnotic). The blood sin- 
uses, sympathetic ganglion cells, and occasional atrophic cortical 
islands occur as heretofore. 
In preparations fixed in Miiller’s fluid, the chromaffin reaction 
of the medulla is always well marked (as in fig. 10). The paren- 
chyma cells appear as brownish masses separated by the un- 
stained vascular areas. There is some variation in the intensity 
of the reaction in different individuals, although the variation is 
not great in sections of the same thickness. It also usually ap- 
pears fairly uniform throughout the medulla, all of the paren- 
chyma cells being somewhat similar in their reaction. Some 
masses or clumps of cells may stain more deeply, however. The 
brownish color appears in the cytoplasm, which may appear 
homogeneous or granular (the granules being most distinct in 
very thin sections). The cytoplasmic vacuoles of various size 
remain perfectly clear and unstained. Whatever their nature, 
they evidently do not contain epinephrin. The nucleus gives the 
chromaffin reaction, being stained slightly darker than the cyto- 
plasm. This was noted by Dostoiewsky (’86) confirming Henle 
(versus v. Brunn). According to Ciaccio (’05), Diamare claims 
that the chromaffin substance fills the whole cell, while Grynfeld, 
localizes it in the cytoplasmic granules. The reaction is weak- 
ened or lost a few hours post mortem (Dostoiewsky, ’86; Ciaccio. 
