SUPRARENAL GLAND—EFFECTS OF INANITION 261 
705, and others), and is also affected by anesthetics (Schur and 
Wiesel, 08; Hornowski, 09). The chromaffin reaction sometimes 
appears also to a variable extent within the blood-vessels of the 
medulla of the rat, as has often been noted in other animals by 
various observers. 
2. Changes in young rats stunted by underfeeding 
Underfed from birth. In arat (St 80.9) underfed from birth to 
twelve days and reaching a body weight of 8.9 grams, the supra- 
renal gland has increased in weight to 0.0026 gram, which is about 
normal for seven days of age. The normal differentiation of 
the suprarenal cortex and medulla has occurred, and the struc- 
ture (in sections stained with hematoxylin) is essentially similar 
to that of the normal rat at seven days. Thus the histological 
differentiation has continued, as in a norma! gland of correspond- 
ing weight, although( as previously shown) the rate of mitosis has 
been greatly diminished. 
In a rat (St 247.5) underfed from birth to seven days, there is 
apparently no increase in the weight of the suprarenal. Frozen 
sections stained with scarlet red and osmic acid show the cortical 
liposomes somewhat similar in appearance to those in the new- 
born, but progressive absorption in the cortical cell strands of the 
medulla is evident. The chromaffin reaction is normal. 
In another rat (St 228.4) underfed from birth to fifty-eight 
days, the suprarenal gland has increased markedly in weight and 
shows a progressive differentiation of liposomes similar to those 
in a normal gland of similar weight (F 7.1). The chromaffin re- 
action in the medulla is variable. Some cell masses show a very 
definite reaction. In others it is entirely absent, giving the 
medulla a spotted appearance. This irregularity of the chro- 
maffin reaction is probably due to the inanition, as it is not ob- 
served in the normal animals. 
Rats at maintenance from three weeks to eight, ten, or twelve weeks 
of age. In these underfed young rats the body weight and supra- 
renal weight (table 1) are but little above the initial weight at 
three weeks. In general, the stained sections (Zenker’s fixation, 
