THE LACHRYMAL GLAND 183 



the center of the cells, they are then much less distinct, at the same 

 time the lumen becomes more obvious. 



The observations of Reicliel, Kolossow, and Lor were also con- 

 fined to the secretory changes. Puglisi-Allegra ('04) contrib- 

 utes an extensive article. 



Studio della glandola lagrimale. ... In una glandola normal- 

 mente funzionante si possona distinguere due specie de cellule. . . . 

 Dopo una eccitazione prolungata e facile notare numerose cellule 

 piuttosta piccole con protoplasma oscura e fortemente granuloso, 

 totalmente prive di secrezione. 



Nicolas saw numerous granules in the lachrymal gland of 

 man after fixation in sublimate or Flemming's solution and stain- 

 ing with aniline-safranin, Biondi-Ehrlich and Altmann's methods. 



Noll saw in the nonstimulated fresh lachrymal gland of the 

 cat ''die meisten Zellen deutlich granulirt. Die Cellgrenzen 

 treten nicht immer gut hervor. " The nucleus — ''der Basis 

 nahe gelegen, als rund oder ovalen." According to him these 

 granules had remarkable differences of refractibility; they dis- 

 appeared when water was drawn under the cover-glass and re- 

 appeared when 2 per cent NaCl was added ; other cells were seen 

 with no apparent granules except numerous 'Protoplasmakorn- 

 chen (these cells he termed 'matte Zellen'); the tissue when 

 fixed and stained by Altmann's method showed faintly and 

 deeply stained cells — ''hellere und dunklere Zellen." Transi- 

 tional cells were also seen which showed both the characteristics 

 of faintly stained and deeply stained cells. Noll fixed fresh 

 tissue with Altmann's fluid and observed the process by means of 

 the microscope. The strongly refracting granules in fresh tissue 

 became the deeply stained granules in prepared sections while 

 the less refracting ('matteren') granules became the faintly stain- 

 ing cells with the network. In the latter case the granules were 

 not conserved but only meshes remained. The deeply and 

 faintly staining cells then represent different stages in the secre- 

 tory activities of the gland, the latter representing the higher 

 stage of granule formation. After electrical stimulation fresh 

 tissue showed ''das die Alveolenbei Weiten nicht so viel Granula- 

 Zellen enthalten. In der Hauptsache sieht man Zellen mit 



