184 JOHN SUNDWALL 



matter Grundsubstanz. " The nuclei were round, granules were 

 present in the neighborhood of the nuclei. 



Fleischer, in the lachrymal gland of the ox, describes pecuhar 

 granular forms — ring, crescent, or demilune granules — which 

 had previously been observed by M. Heidenhain ('90) (See 

 other references to him in Bibliography), Nicolas, and Held. 

 Held, in the submaxillary gland of the rabbit, saw this crescent 

 or ringlike type in addition to the highly and slightly refracting 

 granules. Nicolas failed to observe them in the parotid gland. 

 Heidenhain describes these Halbmondkorperchen in the ac- 

 cessory sex glands of the Triton. The tissue was taken during 

 the heat period. According to him these demilune granules 

 represent a phase in the secretion of the cell; they are formed 

 from small structureless primary granules, and in turn pass into 

 large round secondary granules which are excreted from the 

 cell. . Fleischer found these types of granules in the lachrymal 

 gland of the ox. In sections fixed in 10 per cent trichloracetic 

 acid or picric acid with brillantschwarz-toluidin-blausafranin, 

 he describes (1) round deeply stained small granules, (2) granules 

 of the same size and form which take on a much lighter stain 

 (3) granules with two zones, a dark crescent shaped cap (kapuze) 

 which partly surrounds a Hghter stained trager, (5) oval forms 

 of the type just named, (6) granules in the form of crescents or 

 demilunes without any indications of a trager or lighter stained 

 area, (7) granules with ringlike borders surrounding a lighter 

 stained center. These various types were seen without any 

 special arrangement or distribution. Some cells possessed only 

 the deeply stained round forms, others contained only the demi- 

 lunes, and others again were seen in which all the various forms 

 were present. Fleischer verified his observations by at least 

 four different kinds of fixations in order to escape the possi- 

 bilities of artifacts. He saw also these variously formed granules 

 in the fresh gland. Hence he was positive of their presence. No 

 Halbmondformen granules were seen in the parotid or sub- 

 maxillary gland of the calf. 



Granules in Fresh Tissue. My observations show that gran- 

 ules are readily seen in all fresh lachrymal gland tissue when 



