202 JOHN SUNDWALL 



them as being present in all lachrymal glands and stated that 

 they represent constant and distinct phases in the evolution of 

 the granule. My observations do not agree with those of Fleisch- 

 er. While these peculiar forms of granules were seen occa- 

 sionally in the numerous preparations of lachrymal gland which 

 I made, their occurences were relatively so rare that they must be 

 considered as exceptional. I have also seen these peculiar gran- 

 ules in the accessory lachrymal gland tissue of the third eyelid 

 of the ox as well as in the orbital glands of frogs. Frequently 

 ring granules are seen in fresh preparations of these glands, but 

 the explanation lies in the effect that a large granule may have 

 on light passing through it. Light may pass through the center 

 of the granule with but little or no refraction while it may be 

 greatly refracted at the contour. I think that these peculiar 

 granule structures in fixed preparations can be explained from 

 the standpoint of fixation. It is the large granules which show 

 these peculiarities when present at all. It can be readily con- 

 ceived that the surfaces of these granules may be acted upon 

 more thoroughly by the fixing fluids than the centers and con- 

 sequently stain more deeply. Small tissues well fixed in Bensley's 

 solution or formalin bichromate sublimate solution seldom show 

 these peculiar granule structures. It is difficult to conceive of 

 these granules of fluid or semifluid consistency taking the form of 

 rings, demilunes or crescents. Our knowledge of the physical 

 properties of matter in such condition will not permit of such a 

 conclusion. 



VI. MITOCHONDRIA 



I found that lachrymal gland tissues prepared according to 

 Bensley's method for the demonstration of mitochondria — 

 acetic osmic bichromate, anilin fuchsin methyl green — show these 

 elements very clearly. 



Tubules. In the tubules two chief cell types are made out 

 somewhat similar to those described by Noll in the lachrymal 

 gland of the cat after fixing with Altmann's solution — light and 

 dark cells. 



