212 JOHN SUNDWALL 



kidney after staining with neutral red are not lipoids. Kings- 

 bury used osmic acid and Weigert myelin sheath method. 



For my purposes I found that Herxheimer's stain (Technique 

 III, 5) served best notwithstanding that it stains lecithin, cho- 

 lesterin, and myelin. This however did not materially affect 

 the results. Osmic acid was also used and proved satisfactory 

 but with this fixation it is very difficult to differentiate between 

 the finer lipoids and other structures within the cell. Numerous 

 glands were examined and it was found that the fat content of 

 the cells is subject to much variation. As was pointed out by 

 Bell, fresh glands show more of the finer liposomes than do those 

 after preservation for some time in formalin. 



In some glands numerous droplets of fat were present in all 

 the epithelial elements. The sizes of these varied from some as 

 large as the nucleus to others so fine that they could barely be 

 made out. In these glands the larger fat goblets were generally 

 seen in the base of the cell while the finer ones were located more 

 in the body and proximal portions. There was, however, much 

 irregularity in the distribution. In other glands again the 

 amount of fat present within the epithelial cells was limited to 

 very fine globules irregularly distributed throughout the epithel- 

 ial elements. An abundance of interstitial fat is always seen 

 both in the capsule and the trabeculae. 



In comparing these preparations with similar ones of other 

 glands (pancreas and submaxillary gland of the same animals) 

 the lachrymal gland in the majority of those examined showed 

 very little more fat, if any, in the epithelial cells than did the 

 others. My conclusions then are that frequently the lachrymal 

 gland cells show considerable fat globules but that as a rule the 

 cells in this gland do not possess more than are ordinarily seen 

 in other secreting glands, and that where numerous globules are 

 seen it should be considered in the nature of pathological 

 degeneration. 



Frequently the technique of preparing tissues by frozen 

 method involves, by many, the infiltration with gum arable. 

 The latter stains red in Herxheimer's solution and unless it is 

 completely removed it may simulate fat globules. I was misled 

 by this at first. 



