THE LACHRYMAL GLAND 213 



IX. SECRETION CAPILLARIIOS AND CEMENT SUBSTANCE 



Injection of various masses into the duct system of glands 

 was an early method employed for the demonstration of secretion 

 capillaries. Langerhans, Saviotti, Gianiozzi, Pfliiger, Ewald, 

 and Boll, among others, utilized this means. Objections, how- 

 ever, to this method were advanced by some observers in that 

 the injection mass under pressure produced these capillaries. 

 It was found that Golgi's silver impregnation method demons- 

 strated the secretion capillaries very clearly, as a black deposit 

 is formed within them. Cajal, Retzius, E. Mliller, Langendorf, 

 Laserstein ('94), employed this means. 



While intercellular secretion capillaries for serous glands in 

 general are readily demonstrated by these methods as well as 

 with certain stains much controversy has existed regarding their 

 terminations. Some claim that they enter the cell, intracellular, 

 as in the case of bile canaliculi, while others maintain that they 

 remain intercellular throughout their course. 



Noll described intercellular secretion capillaries in the lachry- 

 mal gland of the feat and Fleischer describes them in the tubules 

 of the lachrymal gland of the ox, of varying lengths — some mere 

 depressions, others almost touching the basal membrane, branch- 

 ing. Hornickel agrees with Fleischer regarding the presence 

 and relations of these capillaries in the lachrymal glands of 

 domestic animals. He further states that none are present in 

 this gland in the pig and dog and explains this condition as being 

 due to the fact that these glands are mucous in character as 

 they react to mucous stains. Puglisi-AUegra demonstrated these 

 structures by Golgi's method. 



For the study of secretion capillaries I used the following: 

 Kopsch-Golgi method (Technique II, 4); vital staining with 

 pyronin (VI, 1) ; and tissues fixed in sublimate alcohol bichromate 

 solution (IV, 2, a) or formalin bichromate sublimate solution 

 (IV, 2, c) stained with iron haematoxylin, copper chrome haema- 

 toxylin, neutral gentian, and neutral safranin (V, 3, 5, 6, 7). 

 My results agree with those of Fleischer and Hornickel. 



THE AMERICAN JOCRNAL OF ANATOMY, vol.. 20, NO. 2 



