224 JOHN SUNDWALL 



6. Neutral gentian — solution of gentian-violet (crystal violet) 

 precipitated by its equivalent of orange-Ci solution (Bensley 

 '11, p. 308). 



7. Safranin-acid violet — precipitate of a saturated solution of 

 safranin O with solution of acid violet (Bensley's 11, p. 309). 



8. Acid fuclisin methyl green — (a) Altmann's acid fuchsin 

 anilin solution: acid fuchsin 20 grams, anilin water 100 cc. and 

 (b) 1 per cent solution methyl green (Bensley '11, p. 309). 



9. Macallum's ('95) iron reaction. 

 VI. Vital staining methods. 



1. Pyronin. (Bensley '11, p. 305.) About eight liters of 

 1-1000 solution in isotonic salt solution injected into the carotid 

 arteries of heads of freshly killed calves: cut arteries in neck 

 clamped off to prevent leakage. Lachrymal gland was deeply 

 stained. By means of the Valentine knife sections 0.5 to 1 mm. 

 were cut and studied with binocular. The acini or tubules 

 stained only hghtly while the ducts, as well as the lumina of the 

 acini including the intercellular secretion capillaries, were deeply 

 stained. The capillaries were best studied by fixing small 

 pieces of this pyronin stained gland in 8 per cent solution of ice 

 cold ammonium molybdate for tweh-e to twenty-four hours, 

 after which they were placed in ice cold 95 per cent alcohol 

 one hour, absolute alcohol one hour, toluol one hour, and paraf- 

 fin one-half hour. Sections were made and capillaries studied 

 with the microscope. 



2. Janus green — one gram in 15,000 cc. of isotonic salt solution. 

 (Bensley '11, p. 305.) Technique same as VI, 1. 



3. Methylene blue — one gram in 10,000 cc. of isotonic salt 

 solution. Technique vsame as VI, 1. 



4. Neutral red — one gram in 15,000 cc. of isotonic salt solution. 

 Technique same as VI, 1. This stain cannot be fixed. 



For detailed consideration of these stains see Bensley ('11). 



XII. -MISC^ELLANEOrS-OTHER FIXATIONS AND SIAIXS 



In addition to the technique outlined and referred to in the 

 general discussion other methods of fixation and staining were 

 employed, the results of which may be briefly summarized as 

 follows : 



