THE LACHRYMAL GLAND 225 



1) Fixations with alcohol failed to preserve the secretory 

 granules and the mitochondria. The cells of the tubules show 

 only the intergranular cytoplasm which appears as a network. 



2) When 5 per cent glacial acetic acid was added to the 70 

 \ier cent alcohol it was found that the granules were partially 

 preserved. In these preparations many of the ring and crescent 

 granules of Fleischer were seen. 



3) The same results outlined in (1) are obtained when C'ar- 

 noy's fixation is used. 



4) The secretion granules are fairly well preserved in picric 

 acid. When stained in the neutral stains of Bensley, one is 

 impressed with the varying intensities to which granules stain. 

 One cell may be filled with the characteristic dark blue granules 

 while the neighboring cell may be fidl of lightly stained yellow- 

 ish granules. The granules in cell type 1 generally show in this 

 fixation greater affinity for the neutral stains than do those in 

 other cells. 



5) Tissues fixed in trichloracetic acid preserved the secretion 

 granules fairly well. However, they did not show great affinity 

 for the neutral stains, foi- after slight differentiation they are 

 but faintly stained. 



It will be recalled that fixations (4) and (5) were used by 

 Fleischer in the demonstration of ring and crescent granules. 

 While these were frequently seen in these fixations they were 

 by no means constant. 



6) Using Kolossow's method, I was unable to demonstrate the 

 epithelial intercellular bridges as described by him. Much 

 shrinkage of tissue was in evidence. 



My experiments with neutral red, vital staining, were not 

 very satisfactory. Four different calves heads were stained by 

 this method and in each instance the gland remained either 

 uncolored or appeared slightly pink. This was found to be in 

 marked contrast to the results obtained in other animals where 

 the gland stained deeply red (monkey). Microscopic exami- 

 nations show that all cells are diffusely but faintly stained. The 

 granules of the tubules and intercalary ducts also appear 

 verv faintlv stained. As elsewhere stated, careful examination 



