76 REPORT OF THE COMMISSIONER OF FISH AND FISHERIES. 
ENDOPLASM. 
Much more coarsely and more or Jess yellowish granular, containing 
numerous nuclei, fat-globules, and sometimes one or more vacuoles; 
also pigment. 
Nuclei.—First observed by Prof. Biitschli' in Myxidium lieberkiihnii, 
where their nuclear nature was shown both by their structure and by 
their affinity for carmine; always very numerous, the smallest occur- 
ring only in the youngest forms, strewn irregularly through the endo- 
plasm. Asin anumber of species the nuclei have been observed to 
originate by division, there is every reason to suppose that such origin 
obtains throughout the subclass’, and that the myxosporidium nuclei 
are to be referred back to the nuclei of the sporoplasm. 
“ Granules” and “ globules..—Many of the structures loosely termed 
“oranules” and “globules” by the older authors are really nuclei, and 
this should be borne in mind in reading their descriptions, which have 
sometimes been reproduced without change (see also pp. 209, 220). 
According to Biitschli (see page 285), these bodies are of a fatty 
nature, as shown by their complete solubility in alcohol. According 
to several other authors, the hematoidin crystals are found within 
: globules whose fatty nature was presumed from the same reaction. 
Thélohan, however (see below), while admitting the solvent action 
of aleohol upon certain chromatophorous globules observed by him 
in Chloromyxum leydigit and in Myxidium lieberkiihnit, denies their fatty 
nature, as osmic acid is without action upon them. 
Fat-globules.—Feebly glittering; size variable; always present except 
in very young individuals; especially frequent in Myxidiwm lieberkiihnii. 
Vacuoles.—Sometimes one or more; number, position, and presence 
inconstant; apparently always nonpulsating. 
Pigment.—Although it has heretofore seemed probable® that all pig- 
ment occurring in the Myxosporidia was of extraneous origin, it would 
appear now, from Thélohan’s recent observations, as though perhaps 
the presence of proper pigment must be admitted. This observer says:? 
In many myxosporidia which live in the free state in the natural cavities one 
finds the endoplasm riddled with strongly colored globules whose tint varies from 
golden yellow to brown. Very numerous in Myxidium, they give to the internal 
face of the pike’s bladder a characteristic yellow tint; they also exist in Chloro- 
myxum leydigii (Mingaz.). As these elements do not resist the action of alcohol or 
that of the essential oils, one finds no trace of them in sections; they are not fatty, 
as osmic acid is without action upon them. , 
Chloromyxum fluviatile also contains similar structures. 
1 Ztschr. f. wiss. Zool., 1881, xxxv, pp. 632-633; Bronn’s Thier-Reich, 1882, 1, pp. 
594-595. Biitschli (1882) was the first to suggest the generality in the Myxosporidia 
of the multinucleate condition. Lankester (see p. 73, foot note 1) took the same view. 
2 This is also Thélohan’s opinion (Bull. Soc. philomat. Paris, 1892, rv, p. 169). 
5 As Biitschli remarked in 1881 (Ztschr. f. wiss. Zool., Xxxv, pp. 642, 649). Cf. also 
Pigment in index. 
