204 REPORT OF THE COMMISSIONER OF FISH AND FISHERIES. 
process of division. Some divide into 2 equal parts (fig. 8); in others the parts are 
of unequal dimensions (figs. 9, 10), and often this division recalls strongly a phe- 
nomenon of terminal or lateral budding (fig. 11). 
Spore.—Very refringent, pyriform; anterior end much more acute; 
length 5to 6 4; shell with very fine longitudinal striz; could not deter- 
mine whether bivalve or not. 
Capsule: In fresh material the highest powers reveal nothing suggest- 
ive of a capsule, the anterior extremity appearing merely more shaded, 
seemingly occupied by ahomogeneous, refringent substance. Onesome- 
times sees, however, near the anterior end, a clear streak (pl. 12, fig.1o) 
believed to be due to thecapsule, but it istoo indefinite and exceptional 
to prove the existence of that structure. Stained sections afford no aid 
here. 
Filaments: Extrusion not produced by iodine, potassium or sodium 
hydrates, glycerin, heat, acetic or formic acids, or by ether. Hydro- 
chloric and nitric acids produced extrusion; thelatter difficultly obtain- 
able, observed only in a very small number of cases in spite of repeated 
efforts. Strangely enough, this method failed completely to produce 
extrusion in 7. octospora and, on the contrary, ether, the only agent 
which succeeded in that species, was without effect on the spores of 7. 
giardi. Filament 15 to 20 4 long; usually extruded completely, some- 
times, however, extruded only partially uncoiled; susceptible to anilin 
stains, among others violet 5B. 
Sporoplasm: Safranin or gentian violet (apparently the best stains 
for these organisms) yield 2 different appearances, according to the 
degree of decoloration. If slightly decolorized, the vacuole alone is 
visible, but when decolorized ad maximum only some colored grains 
remain in front of the vacuole. Sometimes two or three are distin- 
guishable; most frequently, however, only a small colored band (appa- 
rently formed of fused granules of indeterminate number) is seen. 
Vacuole aniodinophile. 
Habitat—Seen only once in Crangon vulgaris Fabr. (shrimp), from 
Boulogne. Probably the course of development is the same as in 
Palemon, asin the single specimen taken the state of development of 
the parasite corresponded to the state of development in Palemon at 
the same date. 
Pathology.—Everything under YT. octospora relative to the opacity 
produced in the host applies equally to 7. giardi, except that, by reason 
of the less perfect normal transparency in, and the pronounced tegu- 
mentary pigmentation ‘of, Crangon vulgaris, the modification is less 
striking, though it is always sufficiently sharp to permit the recogni- 
tion of the infected individuals without any difficulty. 
EH ffects—Ehrenbaum!' noted abnormal individuals of a paler, more 
opaque color, destitute of the normal greenish tone, apparently con- 
siderably enfeebled, dying more rapidly than the normal ones when 
1Zur Nalurgeschichte von Crangon vulgaris, Berlin, 1890, pp. 11, 12. 
oe ee 
