THE MYXOSPORIDIA, OR PSOROSPERMS OF FISHES. 205 
thrown out of the water.” The abnormal individuals never included 
egg-bearing females. 
This, Henneguy and Thélohan think, recalls the aspect of Crustacea 
infected by Myxosporidia. They have also never seen egg-bearing 
females among the infected Palemons. Perhaps we have. here, they 
think, another case of ‘“ parasitic castration.” 
Infection experiments.—A Caradina desmuresti fed for 71 days with 
the muscles of an infected Crangon, showed, on the most careful exami- 
nation, no sign whatever of infection. 
33. Thelohania macrocystis Gurley, 1893. Pl. 12, fig. 3. 
(Sarcosporidian of Palemonetes varians Garbini,' 1891, Rend. Real. Accad. Lin- 
cei Roma, vil, Sem. 1, pp.. 151, 152 with fig.; myxosporidian of ibid., Thélo- 
han and Henneguy, 1892, Compt. Rend. hebdom. Soc. Biol. Paris, rv, p. 586.) 
Thelohania macrocystis, Bull. U. S. Fish Com. for 1891, x1, p. 410; ib., Braun, 
1894, Centralbl. f. Bakt. u. Parasitenkde, xv, p. 86. 
Sporophorous vesicle.—Hlongate fusiform. ‘This is the principal char- 
acter distinguishing this species from T. octospora, which has perfectly 
rounded vesicles. 
Spores.—Hight in number, pyriform, shell difficultly stainable, col- 
oring only in a 0-5 per cent boiling solution of eosin; spores easily 
Stainable by Gram’s method; in the larger posterior end a distinet 
round “nucleus” more clear and transparent than the surrounding 
sporoplasm. ‘Together with these forms are others with a thicker and 
more difficultly stainable shell, within which 8 corpuscles are with diffi- 
culty discernible; probably these represent more advanced stages of 
the same parasite. Garbini failed to find other developmental stages 
corresponding to those found by Henneguy in T. octospora. Inocula- 
tion of healthy animals proved a failure. 
Habitat.—Occurring in great numbers in the muscles of Palemonetes 
varians (prawn) from the Mincio in the neighborhood of Verona. 
Nature——This species has much analogy with Thelohania octospora, 
but presents some noteworthy differences that warrant its specific sep- 
aration. 
Ordo II. Phenocystes Gurley, 1893. 
Etymology: gaivw, Lappear; xvoric, capsule. 
Bull. U.S. Fish Com. for 1891, x1, pp. 409, 410; ib., Braun, 1894, Centralbl. f. 
Bakt. u. Parasitenkde, xv, p. 86. 
Definition.— Myxosporidia, in which the pansporoblast produces few 
(1 or 2) spores; the latter relatively large, with distinct symmetry and 
2 or more capsules;? type family, Myxobclida. 
1 First described in Garbini’s “Intorno ad un nuovo microorganismo parassita del 
Palemonetes varians (title only); Atti Real. Accad. Lincei Roma, 1890, v1, p. 526; 
unpublished. 
2Except Myxobolus unicapsulatus and M. piriformis. This qualification is omitted 
by Braun. : 
