_ THE MYXOSPORIDIA, OR PSOROSPERMS OF FISHES. QA7 
Sporoplasm: In the preparation this had run to a mass with plainly 
visible coarser and finer granules. Sporoplasm traceable only to the 
root of the tail, where its lateral borders converge sharply; in the 
untailed forms it is rounded off posteriorly. 
No nucleus was discovered; bodies staining with hematoxylin, 
borax-carmine, bismarck brown, gentian violet and Hernschwarz were 
resolyed by a Leitz 3, immersion into coarse granule-heaps, having 
little similarity to nuclei. 
Microscopic technique.—Material received fresh; the pathologic mate- 
rial was placed in glycerin and water (equal parts) and fixed with some 
drops of saturated sublimate solution; 14 days later it was transferred 
to 50 per cent, and subsequently to 70 per cent alcohol. In alcohol the 
eggs remained soft. In this form the material was catalogued as Pro- 
tozoa No. 1661 in the collection of the Kénigliches Museum fiir Natur- 
kunde. Bismarck brown stains the capsules only; borax carmine, only 
the sporoplasm. 
Habitat.—Ovary of Lucius lucius (pike) collected at the beginning 
of February, 1892. 
Remarks.—Of Miiller’s forms, the present species resembles most, but 
is not identical with, MW. schizurus. This species also bears a great 
similarity to Lieberkiihn’s figures (in Biitschli) of 1. psorospermicus, but 
here too, specific diiferences exist. On the contrary, he believes the 
present form to be identical with JM. creplini, as the shape and size of 
the two agree well; it is, however, to be noted that the thickness is 
seldom as great as that of the last-named species. 
70. iiyxobolus brevis Thélohan, 1892. 
(Cf. tailed psorosperms of kidney of Gasterosteus acuieatus, Lieberkiihn, 1854, 
Miiller’s Archiv., 1854, p. 357 (see p. 185); myxosporidian spores of G. 
aculectus and G. pungitius (pars) Thélohan, 1890, Annal.de Microgr., 11, 
pp. 198-200, 208, pl. 1, fig. 1; ib. (pars) Thélohan, Compt. Rend. hebdom. 
Soe. Biol. Paris, 11, p. 604.) . 
Henneguya brevis Thélohan, 1892, Bull. Soc. philcinat. Paris, tv, p. 177. 
Myxobolus brevis, Gurley, 1893, Bull. U. 8. Fish Com. for 1891, x1, p, 416. 
Henneguya brevis, Braun, 1893, Centralbl. f. Bakt. u. Parasitenkde, xiv, p. 739. 
Myzxobolus brevis, Braun, 1894, Centraibl. f. Bakt. u. Parasitenkde, xv, p. 87. 
Cyst and myxosporidium not mentioned. 
Spore.—Small; length, 15 4; breadth, 5 to 6 44; anterior portion more 
swollen; tail very short, caudal index hardly 0:50. 
Habitat.—Renal tubules and ovary of Guasterosteus aculeatus (stick- 
leback); renaltubules and ovary of Pygosteus pungitius (9-spined stickle- 
back); all jide Thélohan, letter, 1893. 
Effects.—The following from Thélohan probably refers to this species: 
At the moment of the expulsion it is not rare to see the normal spawning replaced 
by the expulsion of a small mass of gluey and viscous matter in which the micro- 
scopist easily recognizes psorosperms, aborted eggs, etc. 
