262 REPORT OF THE COMMISSIONER OF FISH AND FISHERIES. 
form, becoming globular or more or less ellipsvidal. It is important to 
note that in some individuals the entire protoplasm is transformed, 
changing from globular and yellow to spongy and colorless, the several 
globules disappearing almost in an instant, changing directly into clear 
protoplasm, not growing smaller, as might be thought. This shows 
how rapidly the protoplasm may change its’constitution. Nucleus not 
found either in fresh material or in that treated by hydrochloric or 
acetic acid. Anilin stains only show here and there deeper colored 
granules, which, however, could not have the signification of nuclei. 
Relative to the nuclei, Thélohan, however, says: 
In the myxesporidium of Chloromyxuimn leydigti, as in the other forms, I have beenable 
to prove the presence of numerous nuclei; they are, indeed, of rather small size, but 
nevertheless are easily recognized in sections, and if, as is probable, Mingazzini did 
not observe them, he did not have recourse to this method. 
“ Gregarinoid forms.”—In some gall bladders of the plagiostomes, 
Mingazzini found in summer also other forms of a very different figure, 
which were often united to the myxomycetous forms. These forms were 
uniformly cylindric-elongate, with one end obtusely rounded and the 
other drawn out to a sharp point in the form of a long tail four or five 
times as long as the body, sometimes multiple. Size varying greatly; 
no very small ones seen; large ones equaling the size of adult myxospo- 
ridians. Movements rather rapid, always taking place blunt end fore- 
most. Protoplasm hyaline, or showing round hyaline globules arranged 
in regular longitudinal rows. Many contain a subcentral nucleus. 
Anteriorly the protoplasm contains rather numerous small, strongly 
refracting granules. This form thus resembles a monocystid Gregarine, 
but possesses peculiarities which differentiate it therefrom. For, first, 
an external membrane is wanting, as shown by negative microscopic 
investigation and by the protrusion (in individuals kept for many 
hours on the slide) from the blunt end of thin pseudopodia, which 
bear a great resemblance to those emitted under the same conditions 
by the Myxosporidia; and, second, no known monocystid possesses 
such a whip-like tail. Besides these forms others occur, which, while 
resembling in figure the preceding, have their protoplasm more or less 
charged with yellow granules resembling those of the adult Myxospo- 
ridia. Between these and the Myxosporidia are found other forms 
departing for the most part by more profound alterations of form from 
the first ones. Further, the more advanced gregarinoid forms, which 
possess refracting hyaline globules, take on the character of more 
adult forms, transforming their hyaline globules into yellow globules. 
From what precedes we thus see that the gregarinoi® forms are phases in 
the development of the myxosporidia of the plagiostomes [italics his own]. 
Commentiig upon this view, after noting that Mingazzini remarked 
that these views of the development of the Jyxosporidia (i. e., via the 
“oregarinoid forms”) did not aceord with those held by Lieberktihn and 
Balbiani, Perugia’ says that his own observation of the exit of the 
1 Boll. Scientif., Pavia, 1890, x11, pp. 138, 139. 
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