972 REPORT OF THE COMMISSIONER OF FISH AND FISHERIES. 
Micro-chemistry.—The parasites were studied fresh (by teasing kidney tissue, and 
examining this ina hanging drop, or in fluid media of different kinds), and also 
after treatment with various fixing and staining agents. In the fresh state, a dilute 
solution of potassium hydrate caused a swelling of the spore, and brought out the 
shell and filaments plainly. Glycerin acts well as a medium for the examination of 
thefresh spore. Probably the best medium to use for the hanging drop is toad’s urine. 
Iodine (aqueous solution) colors the spore a uniform brown. In fixing cover-glass 
preparations, no advantage was gained by fixing them in alcohol and ether, or in 
osmic acid, over that obtained by passing the covers through a flame. In the fresh 
state the filaments were made plainer in fixed cover-glass preparations 
[Page 662.] by a number of reagents. Aqueous methyl blue and Babes’ anilin 
water safranin bring the filaments into view quite satisfactorily. 
As fixing agents, Flemming’s solution, Heidenhain’s mercuric chloride solution, 
absolute alcohol, Carnoy’s acetic alcohol, and Perenyi’s fluid were tried, the first and 
last being found unsuitable on account of the production of shrinkage and distor- 
tion. The fixed material was imbedded in xylol paraffin by the usual methods. 
Numerous separate and combined stains were employed with varying results, the cap- 
sules with almost all stains showing the greatest affinity for the coloring matter, the 
degree of affinity varying somewhat in different spores. Pfitzner’s safranin is espe- 
cially good, with a striking affinity for the capsules. Ohlmacher’s dichromophilism 
was demonstrated with fuchsin and iodine green (Russell’s method), and with 
safranin and methyl] blue (Ohlmacher’s method). ‘‘This chromophilous reaction is a 
very striking and possibly significant phenomenon in these organisms.” 
90. Chloromyxum (Sphezerospora) perlatum Gurley, 1893. Pl. 40, fig. 2. 
(Psorosperm of Acerina cernua, Balbiani, 1883, Journ. de Microgr., vu, pp. 201, 
204, fig. 44; ib., Balbiani, 1884, Légons sur les Sporozoaires, p. 133, fig. 40.) 
Myxobolus perlatus, Bull. U. S. Fish Com. for 1891, x1, p. 415; ib., Braun, 1894, 
Centralbl. f. Bakt. u. Parasitenkde, xv, p. 87, 
No description (see also p. 265), 
Habitat.—On Acerina cernua L. 
91. Chloromyxum (Spherospora?) sp.incert. Pl. 40, fig. 3. 
Spore of Lota vulgaris, Biitschli, 1882, Bronn’s Thier-Reich., 1, pl. 38, fig. 22. 
Cyst unknown. 
Myzxosporidium.—Not described. The sporoblast produces a single 
spore?! 
Spore.—Not described. For the reasons given on p. 265, the present 
generic reference of this species is probably the correct one, and the 
species should be closely compared with C. mucronatwm. 
Habitat.—Ovary of Lota lota L. (= vulgaris); ling. 
1“ Hach spore in a special transparent membrane,” 
oY, 
