348 R. A. KOCHEK 



Fatigue cells, 36 measured, average area 0.420 square inches. 

 These results are tabulated along with the measurements of 

 cells from other regions and experiments in table 2. 



LUMBAR ENLARGEMENT OF THE CORD 



Examination of sections stained according to Nissl's original 

 method, e.g. ''seifen methylen blue," after fixation with 95 per 

 cent alcohol, and according to Held's modification of this stain. 

 As regards amount and distribution of chromatic material, there 

 is no difference between the control and fatigue sections. Several 

 sections stained by Held's method showed grouping of cells near 

 the dorsal part of the anterior horns where the chromatolysis 

 was slightly more marked in the fatigue specimen than in the 

 control. This paucity of chromatic substance was not only 

 around the nucleus but also extended to the dendritic trunks. 

 This was not constant in all sections nor throughout the same 

 section. Cells in the extreme anterior horn show no difference 

 from the control. It seems probable that the particular section 

 cut through a nucleus of the cord where the character of the cells 

 was slightly different, since the similar variation in favor of the 

 control cells was observed in some of the subsequent experi- 

 ments. The nuclei of the control and fatigue animals show no 

 difference whatever in the amount and nature of the staining of 

 the chromatic material. 



CRUCIATE GYRUS 



These cells are uniformly stained, the nuclei and nuclear mem- 

 branes and chroniatic bodies being distinct. No difference in 

 the morphology of these cells can be made out with the highest 

 power of the microscope. 



CEREBELLUM 



The cells of the cerebellar cortex stained with Held's method 

 are well defined, and show a distinct architecture. Numerous 

 cells were examined, but without any discoverable difference in 

 the staining reaction between control and fatigue specimens. 



