42 SHINKISHI HATAI 
MATERIAL USED 
The gray snapper, Neomaenis griseus, was chosen for this 
investigation not only because these fish are abundant in sub- 
tropical seas, but also because they possess numerous virtues 
for experimental purposes. The snapper may be kept in the 
laboratory for a long period, and in captivity as well as when 
free, takes almost any kind of food, cooked or raw, animal or 
vegetable. The fish is well known for sagacity and boldness 
and is suited for various kinds of experimentation. Indeed the 
snapper has already been carefully studied by Reighard (08) 
as to its behavior. Thus, with the hope that the gray snapper 
may in future prove to be a suitable form for certain lines of ex- 
perimental work, I have utilized all the brains which have been 
used for chemiéal investigation, together with some others, for 
studying the growth of the brain in weight with respect to body 
length. Most of the fish were secured by netting them, but on 
account of the difficulty of getting the larger fish by this method, 
I have also used dynamite as well as the hook and line. I 
have noted in table 1 the method adopted for catching each 
individual. 
TECHINQUE EMPLOYED 
The fish were examined as soon as they were brought into 
the laboratory. However, as in the case, of netting them, when 
too many were caught at once some were kept in a live box for 
not more than two days, except in a few cases in which they 
were kept for special purposes for several days. When the fish 
were kept in a live box for more than two days it is so stated 
in table 1. 
In every instance the length of body was recorded in the fol- 
lowing way. The fish was laid on its side and the length was 
determined by means of calipers from the tip of the snout to the 
middle of the caudal edge of the tail. The body weights of the 
fish were also taken in a few instances. Although I realized 
the desirability of recording the body weight in all cases, yet it 
was not always possible to make this measurement. 
