140 NAOKI SUGITA 
VI. ON THE NERVE CELLS IN OTHER LAYERS OF THE CEREBRAL 
CORTEX 
Figure 6 shows a diagram of cell-lamination of the adult albino 
rat brain, taken from locality II of the sagittal section (fig. 2, 
Sugita, 17a). In comparison with the data on the pyramids in 
the lamina pyramidalis (IIT) and the ganglion cells in the lamina 
ganglionaris (V), the measurements of the cells found in the 
lamina granularis interna (IV) and the lamina multiformis (VI) 
show nothing peculiar. Generally speaking, the cell body and the 
nucleus of the granules do not take the stain as well as in the ease ° 
of the pyramids and remain rather pale in color. The cells of 
the lamina multiformis, on the other hand, generally stain 
deeply. Especially the cytoplasm of the cells forming the inner 
(ental) sublayer of the lamina multiformis tints very well, so 
that this sublayer is easily distinguished even at a low magnifi- 
cation by the deep staining of the elements. 
The granules in the lamina granularis interna (IV) are smaller 
in size and lie more crowded than do the pyramids. This layer is 
not clearly differentiated in brains weighing less than 0.6 gram or 
less than six days of age, at which stage the immature cells of 
fetal form prevail in both the lamina pyramidalis and the lamina 
granularis interna and no characteristic granules are shown. 
On the sections from a brain weighing 0.5 to 0.6 gram, which had 
been fixed in formaldehyde and imbedded in paraffine, I could 
see distinctly a dark band due to the deep staining of the ground 
substance and characteristic for the adult lamina granularis in- 
terna (ef. Sugita, 717 a, p. 526), though the contained cells do 
not show any of the characteristics of the granules. This is 
probably the first step in the differentiation of the granular layer. 
Later we see that the cells lying near the lamina ganglionaris 
become more and more crowded and somewhat small in size com- 
pared with the cells lying in the lamina pyramidalis. In an adult 
brain weighing more than 1.3 grams, a distinct band of smaller- 
sized cells (the lamina granularis interna) appears above the 
lamina ganglionaris. 
