400 SYDNEY E. JOHNSON 
from the second ganglion of the sympathetic trunk, while figure 
11 is from the fifth or sixth. 
Figure 12 shows a methylene blue preparation of ganglion nine 
or ten (of the same specimen). The autonomic cells and their 
neuraxes are faintly stained as in normal ganglia, but there is a 
total absence of spirals and pericellular networks. No nerve 
endings of any type could be found. ‘ 
A pyridine silver preparation made from a posterior ganglion of 
the left trunk of the same specimen (spec. 27) is shown in figure 
14. It supports the results obtained with methylene blue. 
Specimens 28, 29, and 37 require a word of explanation. 
Dissection, after injection, disclosed the fact that the sympa- 
thetic trunks had been divided on one side only in specimens 28 
and 29. Spirals and networks were easily demonstrated in the 
- ganglia of the trunks which had not been divided, but could not 
be found in the posterior ganglia of the divided trunks. 
Both sympathetic trunks were missed in specimen 37. The 
ganglia were stained, however, and they gave results identical 
with the results obtained on the first two lots of frogs in which 
the spinal cords only were destroyed, indicating that the devital- 
izing effect of the rather severe operation had no detrimental 
influence on the staining properties of the nerve terminations. 
IV. SUMMARY AND CONCLUSIONS 
The results obtained in the three sets of experiments may be 
summarized in tabular form as follows: 
