96 THE ATLANTIC. [cHaP. I. 
28. Examine mud brought up by dredge from different depths for 
living Diatoms; examine also for the same purpose the stomachs of 
Salpe and other marine animals. 
29. Note algze on ships, etc., with the submerged parts in a foul con- 
dition ; also preserve scrapings of colored crusts or slimy matter, green, 
brown, ete. 
30. Observe algze floating, collect specimens, noting latitude and lon- 
gitude, currents, ete. 
31. Examine loose floating objects, drift-wood, etc., for algae. If no 
prominent species presents itself, preserve scrapings of any colored 
crusts. Note as above. 
32. It might be useful to have a few moderate-sized pieces of wood, 
oak, etc., quite clean at first, attached to some part of the vessel under 
water to be examined, say, monthly. The larger or shorter prominent 
alow should be kept and noted, and crusts on such examined and pre- 
served, with notes of the vessel’s course. 
33. Various instances have been mentioned by travelers of the color- 
ation of the sea by minute algze, as in the Straits of Malacca by Harvey: 
any ease of this kind would be worth especial attention. 
34. The calcareous alge (Melobesia, etc.) are comparatively little 
known, and are apt to be overlooked. 
35. Fresh-water algae should be collected as occasion presents. Pro- 
fessor Dickie states that they may be either dried like the marine kinds, 
or preserved in a fluid composed of three parts alcohol, two parts water, 
one part glycerine, well mixed. 
36. Cases are recorded of the presence of alge in hot springs. If 
such are met with, the temperature should be noted and specimens pre- 
served. 
LV. ZooLoGicaL OBSERVATIONS. 
As the Scientific Director of the expedition is an accomplished zool- 
ogist, and has already had much experience in marine exploration, it 
will suffice to offer a few suggestions under this head. 
The quadrant-like zone of the Pacific, which separates the northern 
and eastern boundaries of the Polynesian Archipelago (using ‘ Polyne- 
sia” in its broadest sense as inclusive of ‘“ Micronesia”) from the coasts 
of N. Asia and America, is as little explored from the point of view of 
the physical geographer as from that of the biologist. It would be a 
matter of great importance to examine the depth, and the nature of the 
