XViil JoURNAL OF COMPARATIVE NEUROLOGY. 
lemma becoming continuous with the sarcolemma, and breaks up inte 
fibrils which pass into the granular substance but do not pass beyond it 
to enter into relation with the contractile substance of the fiber. The 
second class is represented by FOETTINGER, who also recognizes a 
Doymre’s elevation under the sarcolemma, containing numerous nuclei. 
The terminal branches of the nerve fiber, however, according to Fo- 
ETTINGER, pass through the granular substance and out in different di- 
rections, finally fusing with the isotropic or intermediary disk of the 
muscle fiber. This, then, represents the theory that there is a direct 
anatomic continuity between the nerve fiber and the muscle fiber. 
The author’s investigations were made on Hydrophilus piceus and 
Melolonta vulgaris. He used the haematoxylin method suggested by Dr. 
C. Necro in 1889. The fresh muscles of the wings and legs of the living 
insects were immersed for 24 to 48 hours in DELAFIELD’s haematoxylin 
solution, washed thoroughly in water, decolorized in a weak acid mix- 
ture of glycerine, water and hydrochloric acid. They were then teased 
and mounted in a medium consisting of equal parts of glycerine and 
water. 
In the insects studied, the motor nerve undergoes dichotomous 
division of the axis cylinder, the two branches diverging 
and entering a mass of granular substance which has in profile the. 
form of a cone slightly elevated above the level of the muscle fiber. 
In the interior of the granular substance, which is stained more or less 
intensely violet, he finds three or four nuclei which appear granular 
and stain deeply, resembling the telolemma nuclei described by 
K tHNE in the motor plaques of vertebrates. He was unable however 
to find the so-called sole-nuclei also described by KUHNE. 
While the author has, by this method, confirmed the findings of 
other investigators regarding the existence of a Doykre’s elevation of 
granular substance containing nuclei, similar to those found in verte- 
brates, he has as yet been unable to establish several of the most im- 
portant points in dispute as to the structure of the motor ending in the 
striated muscles of insects. He has been unable to determine whether 
the cone-shaped eminence described by him is under the sarcolemma 
or not and he is not certain from his preparations that the nerve fibrils 
form in the granular substance a terminal arborization similar to that 
found in the motor endings in the striated muscles of vertebrates. 
These points, with the minuter structure of the Doyérk’s elevation, he 
reserves for further investigation. 
The main interest of the research seems to have centered in the 
question whether the fibrils end in the Dovkre’s elevation or pass be- 
