204 
JouRNAL OF ComPaARATIVE NEUROLOGY. 
preparations were in many cases practically as good as 
those that were re-mordanted, there being only a slight 
diminution in the intensity of the stain. 
D. Cerebrum of infant. Hardened in formalin as described above. 
Imbedded in celloidin without mordanting. Sections made 
were treated as follows: 
I 
Copper acetate 46 hours. A.h.1% hours. Osmic. Pal. 
Not good, fibers very pale. 
Copper acetate 46 hours. A.h.1% hours. Borax-ferri- 
cyanide. Not good. 
Copper bichromate 46 hours. A.h.1% hours. Osmic. 
Pal. Not good. Somewhat better than 1. 
Copper bichromate 46 hours. A.h. 1% hours. Borax- 
ferricyanide. Not good. 
Copper bichromate 46 hours. A.h 1% hours. Osmic. 
Borax-ferricyanide. Not good. 
Copper bichromate 46 hours. A.h. 47 hours. Osmic. 
Pal. Better than any of preceding. 
Copper bichromate 46 hours. A.h. 23 hours. Osmic. 
Pal. Like 6. 
Copper bichromate 46 hours A.h. 23 hours.  Borax- 
ferricyanide. Not good. 
Even the best of the above were too pale, perhaps partly, 
but hardly principally, attributable to the youth of the 
brain. 
E. Cord and medulla of infant 1% months old. Hardened in form- 
alin as above described. Pieces mordanted i” foto, 
immediately after removal from formalin, in copper 
bichromate 3% for seven or eight days. Imbedded in 
STEPANOW’S Clove oil celloidin.! 
Sections were treated as follows: 
No further mordant. A.h. 12 hours. Osmic. Pal. Good. 
No further mordant. N.h. 16 hours. Osmic. Pal. Very 
good. . 
Copper bichromate 4 hours. N.h. 15 hours. Osmic. Pal. 
Very fine, better than 1 and 2. 
Copper bichromate 18 hours. N.h. and A. h. 6 hours. 
Osmic. Pal. Slightly inferior to 3. 
1 Zeitschrift fiir wiss. Mikroskopie, BA. XVII, H 2, 1900. 
