StronG, Technique .of Wergert's Method. 295 
5. Copper bichromate 18 hours. N.h., kept slightly warmed, 
2to4 hours. Osmic. Pal. Very fine, possibly some- 
what better than 3. 
F. Cord, medulla and basal ganglia of child 3 years and 44% months 
old. Hardened in formalin as above described and pre - 
served in formalin for about three years before being 
used. 
(a) Pieces of cord mordanted in copper bichromate 3% seven days. 
Imbedded in clove oil celloidin. Sections treated as 
follows : 
1. No further mordanting. H. Osmic. Pal. Good. 
2. Copper bichromate. H. Osmic. Pal. Better than 1. 
3. Potassium bichromate-+ osmic. H. Osmic. Pal. Not good. 
(2) Pieces of cord mordanted in potassium bichromate + osmic. 
Sections made as in (a) and treated as follows: 
1. No further mordanting. H. Osmic. Pal. Much better 
than (a2) 3 but not nearly as good as (a2)1 or 2. Med- 
ullary sheaths of large fibers appear to be better fixed, 
however, than in (a). 
2. Potassium bichromate +osmic. H. Osmic. Pal. Better 
than (6) 1. 
3. Copper bichromate. H.Osmic. Pal. Very good, not so 
brilliant as (a) 2 but the fibers, especially in the white 
matter, look better than in (a). 
(c) Pieces of cord mordanted seven days in potassium bichro- 
mate 5 + chrome alum 2+ water 100. Sections made 
as in (a): 
1. No further mordanting. H.12 hours. Osmic. Pal. Fair. 
(a2) Pieces of cord mordanted seven days in potassium bichro- 
mate 5%. Sections made as in (a). 
1. No further mordanting. H.12 hours. Osmic. Pal. Not 
as good as (c). 
(ec) Pieces of cord mordanted seven days in copper bichromate 
3%. Sections made as in (a). 
1. No further mordanting. H.12 hours. Osmic. Pal. Fair, 
a little better than (c). 
(f) Pieces of cord mordanted seven days in potassium bichro- 
mate + osmic. Sections made as in (a). 
1. No further mordanting. H.12 hours. Osmic. Pal. Fair. 
(g) Pieces of cord mordanted seven days in iron alum 4%. 
Sections made as in (a). 
