NERVUS TERMINALIS: MAMMALS 13 



1 sagittal series through forebrain and nasal septum of kitten two weeks old, 



pyridin-silver method. 



2 sagittal series of nasal septum of kittens two weeks old, pyridin-silver method. 



1 sagittal series through forebrain and nasal septum of kitten one day old, fixed 



and decalcified in Zenker's fluid and stained by the Weigert method. 



2 septal mucosae of kitten one day old, stained with methylene-blue. 

 Numerous series of pig embryos at various stages, stained with haematoxylin or 



with Mallory's connective tissue stain. 



In addition to these sections, the following materials were also 

 studied according to the method indicated: Numerous dissec- 

 tions of kittens, of puppies, and of adult dogs and cats were made. 

 The method described by McCotter, by which the head is fixed 

 in Miiller's fluid to which acetic acid has been added, was used 

 with good results. Fixation in 10 per cent formalin, followed by 

 decalcification in 10 per cent nitric acid, was also adopted in 

 many instances. A light surface stain with borax-carmine was 

 found advantageous in differentiating the nervus terminalis 

 from the neighboring tissues. 



Frozen beef brains were obtained from the Chicago Stock 

 Yards, and were found to be very favorable for the dissection of 

 the delicate strands of the nervus terminalis. These refriger- 

 ated brains were allowed to thaw in a solution of 10 per cent 

 formalin at room temperature. Besides being relatively easy to 

 dissect, this material responded well to the gold-chloride tech- 

 nique and gave excellent histologic preparations. A number of 

 beef fetuses of 110 mm. to 140 mm. greatest length were also 

 dissected. These had been preserved in formalin. Supple- 

 mentary studies were made on beef material from which the 

 meninges and a portion of the brain beneath the region of the 

 nervus terminalis were removed and fixed in 1 per cent osmic acid, 

 immediately after the brain was taken from the cranial cavity. 

 Most of the material thus obtained was still warm when fixed. 

 It was made possible to obtain this through the courtesy of 

 Swift & Company. 



The brain of a full-term mule, freshly removed and fixed in 

 10 per cent formalin, was obtained. Another mule fetus of 121 

 mm. greatest length was also studied. The brain, in situ, and 

 the nasal septum of an adult horse was obtained through the 



