264 KIYOYASU MARUI 



the same category of nuclear change; we observe here besides the 

 nucleohis a rod-shaped substance which shows a staining reac- 

 tion similar to that of the nucleolus, although I am not sure 

 about this, as it is from a Levaditi preparation. As far as I know, 

 no such picture of the nucleus has been described before; figure 8 

 of Dolley's (7) publication demonstrates a cell, the nucleus of 

 which shows a deeply stained spheroidal substance besides the nu- 

 cleolus, but Dolley did not mention anything about that in the 

 text or in the description of the plate. Whether this kind of mani- 

 festation of the nucleus is to be regarded as the alteration of double 

 nucleolus, which latter is ir et not infrequently, or can be attrib- 

 uted to a special appearance of the nucleus in fatigue, I can- 

 not tell. 



Holmgren (17), Sjoeval (32), and others found the stainable 

 substance massed about the nuclear membrane, and forming either 

 an irregular or a complete ring. I also observed the same phenom- 

 enon in many cases (fig. 3) ; the nuclear membrane was out- 

 lined by a delicate blue-stained line or a large mass of stain- 

 able substance in a different portion of its circumference, giving 

 the picture of a half-moon. Sjoeval and Holmgren interpreted 

 this as a restitution phenomenon of the tigroid substance; Dolley 

 (7) also regarded it as a sign of greater nuclear activity. I agree 

 with the opinion of these authors. Holmgren (17) observed besides, 

 that the nucleolus and the nuclear granulation emigrate from the 

 nucleus into the cell body. The emigration of the nucleolus never 

 came to my observation; the case, however, from which figure 4 

 was reproduced mjiy indicate the emigration of stainable sub- 

 stance from the nucleus into the cell body. The nucleus as well 

 as the cell body is swollen in this case, and the nucleolus is also ex- 

 tremely swollen, and we find many blue-stained granules going 

 out of the nucleus into the cell protoplasm. On the other hand, 

 I found also the accumulation of the acidophile substance in the 

 nucleus (fig. 5). On the basis of these findings I should agree 

 also with Holmgren, who came to a conclusion that a mutual inter- 

 change of substance takes place between nucleus and cell proto- 

 plasm in activity. On the ground of Richard Hertwig's doctrine 

 of nucleus-plasm ratio, Dolley (6, 7, 8, 9) measured the size of cell 



