268 KIYOYASU MARUI 



Some glia cells even lie freely in and about the synapse; to this I 

 shall come back again. 



In the thionin-eosin preparations of the formol-Zenker material 

 it is very difficult to find such delicate alterations of the reticular 

 structure. In a slight alteration it is almost impossible to distin- 

 guish the difference between the resting condition and the fatigue 

 case, so far as the net figure is concerned. In the most advanced 

 stage of alteration, however we are able to find similar manifesta- 

 tions as those described in Levaditi preparations, although it is 

 not so easy to see as in these preparations. Figures 2 and 5 were 

 reproduced from thionin-eosin preparations. In figure 2, which 

 demonstrates the resting condition, we find regularly arranged 

 dark points around the cell, which evidently represent the net 

 beams of the pericellular reticulum. In the fatigued cell (fig. 5) 

 we find around the cell body a number of similar dark points, which 

 are, however, scattered without any order at the cell periphery. 

 The microscopic observation revealed clearly a change of peri- 

 cellular network similar to that described in Levaditi prepara- 

 tions; the net beams were in part swollen and others broken up. 

 The findings of the glia cells I shall describe later. In the Heiden- 

 hain preparations and other preparations a similar manifestation of 

 the reticular structure was observable, although it is not so clearly 

 demonstrable as in the Levaditi preparations so that we can find 

 the alteration only in a far advanced state. 



B. Manifestations of the 7ieuroglia cells in the synapse 



Among the neuroglia cells of the synapse of the Mauthner cell 

 I found in many cases Alzheimer's amoeboid glia cells (figs. 9, 10, 

 11, 12, and 14). These amoeboid glia cells, as characterized by 

 Alzheimer (1), show a large protoplasmatic cell body with a spe- 

 cial morphological structure and small dark nuclei, and in typical 



Figs. 0, 10, and 14 Thionin-eosin preparation (fatigued). 

 Fig. 11 Acid-fuchsin-light green preparation (fatigued). 

 Fig. 12 Mallory preparation (fatigued). 

 Fig. 13 Scharlach R stain (fatigued). 



