468 J. M. D. OLMSTED 



CONCLUSIONS 



It is evident, therefore, that the growth of the nerve into the 

 appropriate region precedes the appearance of taste-buds, and 

 that the formation of dermal papillae, the immediate forerunners 

 of the taste-buds, is most intimately connected with the growth 

 into it of the particular branch of the nerve trunk which is to 

 innervate it. 



SUMMARY 



1. The nerve and cartilage in all stages of regenerating ends of 

 barbels of the catfish, Amiurus nebulosus, extend practically the 

 complete distance from the old stump to the basement membrane 

 of the epidermis at the very tip. 



2. Short regenerated pieces, though possessing cartilage and 

 nerve, show no trace of taste-buds. 



3. The formation of dermal papillae, the invariable forerunners 

 of taste-buds, takes place at the base of longer regenerated pieces 

 as if the germinative layer of the epidermis were indented by the 

 growth into it of a small branch from the nerve trunk. 



4. Since taste-buds degenerate in a barbel whose nerve is cut 

 and reappear when the nerve regenerates, and since the nerve 

 appears in the appropriate region before there is any evidence of 

 a developing taste-bud, the presence of the nerve may be said 

 to be the causative factor in the formation of taste-buds. 



BIBLIOGRAPHY 



Hermann, F. 1884 Beitrag zur Entwicklungsgeschichte des Geschmacksorgans 

 beim Kaninchen. Arch. f. mikr. Anat., Bd. 24, S. 216-229. 



Land acre, F. L. 1907 On the place of origin and method of destribution of taste- 

 buds in Amiurus melas. Jour. Comp. Neur., vol. 17, pp. 1-67. 



Mallory, F. B., and Wright, J. H. 1915 Pathological Technique. Pliila. 

 ~>'.'A) pp. 



Marchand, M. L. 1902 Developpement des papilles gustatives chez de foetus 

 humain. Cont. Rend. Soc. Biol. Paris, T. 54, pp. 910 912. 



Moral, II., dnd Hoseman, (1. 1919 Uber den Einflusa der Nerven auf das 

 Wachstum der Zahne. Anal. Hefte, Bd. 57, S. 201-250. 



Szymonowicz, W. 1895 Beitrage zur Kenntniss der Nervenendigungen in Haut- 

 gebilden. Arch. f. mikr. Anat., Bd. 15, S. 624 654. 

 L896 I Clicr lien l',:ni mid die Knt wickelung der Nervenendigungen in 

 Entenschnabel. [bid., Bd. 48, S. 329-358. 



