cxxxviii Journal of Comparative Neurology. 



A Rapid Modification of Weigert's Method. 1 



Dr. Azoulay has devised a modification of Weigert's method of 

 staining for which he claims excellent results and very great economy 

 of time. It is applied to the axial nervous system as follows : — Hard- 

 en three or four months in bichromate, or little pieces more rapidly in 

 a mixture of bichromate of potash 3 per cent., 100 parts, and osmic 

 acid 1 per cent., 25 to 30 parts; imbed in parafhne (or collodion or 

 any other suitable medium ); section in 90 per cent, alcohol; free the 

 sections of paraffine with xylol or benzine ; treat with absolute alcohol; 

 apply Schweitzer's reagent (ammoniacal cuprous oxide undiluted) 

 three to five minutes according to the thickness of the sections; wash 

 by agitating in distilled water about 30 seconds; treat with hematoxy- 

 lin (1 part to 10 of alcohol and 90 of water) either several days old, 

 or fresh and treated with a drop of saturated solution of carbonate of 

 lithium, or merely ordinary hasmatoxylin with alum, three to six min- 

 utes according to the thickness of the sections; the sections are to be 

 heated on the slidft while bathed in the stain to 6o° ( pass the slide 

 over a flame until it steams, repeat the heating three or four times, 

 taking care that the preparation floats in the fluid free from the slide); 

 wash in water ; treat with Weigert 's decolorizer, prussiate of borax, 

 5, 10, 15, or 20 minutes according to the temperature employed and 

 the thickness of the sections ; arrest the decoloration when the large 

 cells have only their nuclei colored a very dark brown ; wash care- 

 fully in water ; mount in acetone and balsam in benzine or xylol. 

 The decoloration is so slow that one can be secure from decolorizing 

 more than is necessary. The sections keep at least two years and a 

 half. 



Course of the Fibres from the Striatum 2 . 



In 1887 Dr. Edinger showed that in all vertebrates a thick bundle 

 arises from the striatum, passes caudad to the thalamus where a part 

 ends in a large nidus, while the remainder extends still further caud- 

 ad. One bundle of the latter passes to the regio infundibuli, the 

 course of the remainder was not determined. To solve this question 

 three methods were employed, which gave concordant results; (1) the 



1 Azoulay, L. Methode de coloration de Weigert rapide et transparente. 

 Bui. Soc. Anat. de Paris, 5 Ser., VIII, 10, 1894. 



2 Edinger, L. Die Faserung aus dem Stammganglion Corpus Striatum. 

 Vergl.-anat. und experimentell untersucht. Verhdl. Anat. Gesellschaft, May, 

 1894. 



