102 HENRY O. FEISS 
8. Measurements. Circumferences above paw, ankle and knee 
9. Reflexes: knee jerks; anal reflex (observed by inserting glass rod into anus) 
10. Control of sphincters (based on ordinary clinical observations) 
11. Response to faradism; applied to various parts of limb 
12. Remarks 
13. Photograph 
In a few of the dogs data bearing on spinal localization could 
be obtained by direct stimulation of peripheral nerves, which 
was done at the time of the secondary operations of nerve fusion. 
Study of autopsy material 
The spinal cords were studied in twenty of the cases. In six 
of them proper identification of the roots could not be made, 
owing to scar. The other fourteen were each mounted on card- 
board, and the roots spread out, stitched down, and properly 
numbered. The count was made from the thirteenth, using the 
last rib as a land-mark. Each mounted specimen was sketched 
and placed in Miiller’s fluid. 
The boundaries of the cord segments were usually estimated 
by means of their relations to dural root exits, this relationship 
having been based on a number of dissections of normal cords. 
After proper hardening, the cord was cut transversely into a 
number of pieces without attempting to remove the dura, so as 
not to disturb the rootlets within. The exact location of each 
of these cuts in relation to dural root exits was indicated on the 
sketch. The pieces were cut 2 to 3 mm. thick and imbedded in 
celloidin. Sections from each piece were stained by at least three 
methods, Hematoxylin-eosin, Van Gieson and Weigert-Pal. 
PART 1. CLINICAL IMPROVEMENT AFTER THE PRODUCTION OF 
THE LESION IN RELATION TO THE HISTOLOGICAL 
FINDINGS AT DIFFERENT STAGES 
This portion of the research is based on the studies of twenty 
dogs and their spinal cords. The important points are summa- 
rized in table 1. Glancing at this, it is seen that after such a 
lesion as described, almost all the animals showed more or less 
clinical improvement. It is further seen that this improvement 
