OLFACTORY CENTERS IN TELEOSTS 181 
xylene, embedded in paraffine and sectioned at 5 micra. This 
method is of particular value in tracing the course of the nervus 
terminalis. 
V. Gold chloride method 
This was used to bring out the unmedullated fibers, particu- 
larly in the olfactory bulbs; it is not of great value, however, owing 
to its low penetrating power. The method of fixation in formalin 
as given in Hardesty’s ‘Neurological technique,’ was followed with 
the best results. Two series of sections of the bulbs were pre- 
pared by this method. 
VI. Golgi method 
Individuals two to five centimeters in length. 
Five series transverse sections. 
One series frontal sections. 
Individuals twenty-five to forty centimeters in length. 
Six series transverse sections. 
One series sagittal sections. 
Nine series cut in various oblique planes. 
Fourteen series of the olfactory bulbs cut in various 
planes. 
Two different methods were followed, both of which gave good 
results. With most of the Golgi material the fish were killed, the 
brains removed and placed for three to five days in a mixture of 
two parts of 3 per cent aqueous solution of potassium dichromate 
and one part of 1 per cent aqueous solution of osmic acid. Next 
they were rinsed in a ? per cent solution of silver nitrate in which 
they may remain indefinitely but are ready for dehydration and 
embedding in celloidin after two to four days. The second method 
was used for brains fixed in 4 per cent formaldehyde. 
After fixation for forty-eight hours the brains were washed in 
running water for twenty-four hours and then placed in a 3 per 
cent aqueous solution of potassium dichromate at a temperature 
of 40° C. for six to ten days. They were next placed in the 
osmium-dichromate mixture and treated as outlined for the first 
