124 Journal of Comparative Neurology. 



very cheap and inexpensive. In the other methods we must 

 use cover shps, because the high magnification which they re- 

 quire — and then one does not see much — would not be apphcable 

 with the thick layers of damar. We do not require this and 

 thereby escape not only expense but also the difficulty of 

 avoiding bubbles of air under large coverslips whereby the pre- 

 paration is often endangered.' 



"It appears to me, apart from all economy of material, 

 time and labor, as well as the convenience of cutting pieces in 

 the microtome so to speak at odd moments without injury to 

 them from the long contact with water, that this method which 

 enables us for the first time to follow in sections the course of 

 nerve fibers through the whole brain shows an advance in the 

 technique of the study of the central nervous system and takes 

 precedence over all others. 



"As I pass over the application for the macroscopical study 

 of the brain which Dr. Mondino has also made of this method, 

 I will here in conclusion again assert that the sublimate method 

 takes a high place among the microscopical methods for the 

 study of the nerve centers, alongside of the methods in which 

 silver nitrate plays the chief role." 



Additional technical notes in Golgi's article, "Das diffuse 

 nervose Netz der Centralorgane des Nervensystems. Seine phy- 

 siologische Bedeutung " (from the Rendiconti des R. Instituto 

 Lombardo, Sen II, Vol. XXIV, Fasc. 8 and 9), pp. 259 and 

 260 ot the German edition of Golgi's works : 



"The method which was most useful to me in the investi- 

 gations described in the first part of this work, was the staining 

 of the nervous elements with mercury sublimate, but with a 

 modification which enhanced its demonstrative value without 

 changing the fundamental procedure. The latter consists (i) 

 in the hardening of the pieces in bichromate of potassium, 

 (2) in the transference from this into a ^ % to i % solution of 

 bichloride of mercury. 



" Since I have given in another work (Studi sulla fina ana- 

 tomia degli organi central! del sistema nervoso, p. 202) a detailed 

 description of what I call the fundamental part of the method, I 



